| 金钗石斛优良种源的适生条件及仿野生栽培的关键技术 |
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| 引用本文： | 龚记熠, 李菲, 孙威, 等. 内生真菌对营养亏缺下金钗石斛幼苗生长的影响[J]. 云南农业大学学报（自然科学）, 2019, 34(1): 110-115. DOI: 10.12101/j.issn.1004-390X(n).201801029 |
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| 作者姓名： | 龚记熠 李菲 孙威 唐婧 乙引 |
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| 作者单位： | 1.贵州师范大学，国家林业局西南喀斯特山地生物多样性保护重点实验室，贵州省植物生理与发育调控重点实验室，贵州 贵阳 550001 |
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| 基金项目： | 贵州省科技合作计划项目（黔科合LH字[2015]7764号）；贵州省教育厅“125”重大专项（黔教合重大专项字[2012]005号）；教育部创新团队发展计划项目（IRT1227）资助 |
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| 摘 要： | ttttt目的ttttt探讨营养亏缺下内生真菌对金钗石斛组培苗生长的影响。ttttttttttttt方法ttttt金钗石斛组培苗移栽时在其根部分别添加内生真菌37号、117号、120号菌株及无菌水，然后再分别在1倍、1/2倍、1/4倍和1/8倍Hoagland营养液中培养30 d，通过测定植株生长特征、生理特性等指标来判定内生真菌在不同稀释倍数营养液中的作用效果。ttttttttttttt结果ttttt随着Hoagland营养液逐渐变稀，各内生真菌处理组的植株干重、叶绿素SPAD值、总生物碱、水溶性多糖和氮磷钾含量增加量显著高于对照组，根长和根茎比增加量显著小于对照组。3株内生真菌处理间各种指标变化并不完全相同，37号菌株在增加干重和总生物碱含量上作用最为显著，而120号菌株在控制根长和根茎比、提高水溶性多糖含量、增加对磷和钾元素的吸收上效果更为突出，117号菌株在提高叶绿素SPAD值和促进植株吸收氮元素上作用最为明显。ttttttttttttt结论ttttt3株菌株均能促进金钗石斛幼苗在营养亏缺下的生长，但作用效果各不相同，生产中混合使用效果更佳。tttt
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| 关 键 词： | 金钗石斛 内生真菌 营养亏缺 生长特性 |
| 收稿时间： | 2018-01-19 |
| 修稿时间： | 2018-05-02 |
Biomass yield,mineral content,and nutritive value of Poa pratensis as affected by non-clavicipitaceous fungal endophytes |
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| Jiyi GONG, Fei LI, Wei SUN, et al. Effects of Endophytic Fungi on the Seedling Growth Characteristics of Dendrobium nobile under Nutritional Deficiency[J]. JOURNAL OF YUNNAN AGRICULTURAL UNIVERSITY（Natural Science）, 2019, 34(1): 110-115. DOI: 10.12101/j.issn.1004-390X(n).201801029 |
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| Authors: | Jiyi GONG Fei LI Wei SUN Jing TANG Yin YI |
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| Affiliation: | 1.Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountain Area of Southwest of China, Key Laboratory of Plant Physiology and Developmental Regulation, Guizhou Normal University, Guiyang 55001, China |
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| Abstract: | tttttPurposetttttThe effects of No.37, 117 and 120 endophytic fungi strains isolated from Dendrobium nobile on the growth and physiological characteristics of D. nobile under nutritional deficiency condition were detected in this study.ttttttttttttttttttMethodtttttThe endophytic fungi strains No. 37, 117, 120 and distilled water were sprayed on the root of tissue culture regenerated seedlings of D. nobile. Each group was cultivated for 30 days with 1, 1/2, 1/4 and 1/8 of Hoagland nutrient solution, respectively. After 30 days, the effect of endophytic fungi inoculation was determined by measuring the plant growth and physiological characteristics.ttttttttttttttttttResultstttttWith the decrease of Hoagland solution concentration, the endophyte treatment groups were significantly higher than the control group (treated with distilled water) in dry weight, chlorophyll SPAD value, total alkaloids, water-soluble polysaccharides and NPK content. The root length and root shoot ratio increased less than the control group. The changes of various indexes were not completely the same between the three endophytic fungi strain treatments. Strain No.37 treatment showed the most significant ability in dry weight and total alkaloid content increase. Strain No.120 was more prominent in controlling root length and root shoot ratio, promoting water-soluble polysaccharide content and phosphorus and potassium absorption. Strain No.117 showed the most obvious effect on increasing chlorophyll SPAD value and promoting nitrogen absorption.tttttttttttttConclusiontttttAll of these three endophytic fungi strains can promote the growth of D. nobile seedlings under nutrient deficit condition, but they showed different types of effect. Therefore, the combination use of these three endophytic fungi will produce better results.ttttttttt |
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| Keywords: | Dendrobium nobile endophytic fungi nutritional deficiency seedling growth characteristics |
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