| 生物炭–过氧化钙复合颗粒对酚酸胁迫下番茄生长和根际微生态的调控效应 |
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| 引用本文： | 涂玉婷,彭智平,许杨贵,李珠娴,黄继川. 生物炭–过氧化钙复合颗粒对酚酸胁迫下番茄生长和根际微生态的调控效应[J]. 植物营养与肥料学报, 2022, 28(10): 1914-1927. DOI: 10.11674/zwyf.2022292 |
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| 作者姓名： | 涂玉婷 彭智平 许杨贵 李珠娴 黄继川 |
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| 作者单位： | 广东省农业科学院农业资源与环境研究所 / 广东省养分资源循环利用与耕地保育重点实验室 /农业农村部南方植物营养与肥料重点实验室，广东广州 510640 |
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| 基金项目： | 国家自然科学基金项目(41701341，42177337)；广东省自然科学基金面上项目(2019A1515011737， 2022A1515010753)；广东省现代农业产业技术体系创新团队项目(2019KJ140)；广东省农业科学院中青年学科带头人培养项目(R2020PY-JX021)。 |
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| 摘 要： | 【目的】酚酸胁迫是造成番茄连作障碍的主要因素之一。本研究探讨了生物炭–过氧化钙复合颗粒(简称复合颗粒)作为土壤改良剂缓解番茄酚酸胁迫的效果和机理。【方法】供试番茄品种为千禧圣女果，供试土壤为赤红壤。盆栽试验共设5个处理：常规栽培(CK)，酚酸胁迫处理(T1)，添加外源酚酸的同时分别添加生物炭–过氧化钙复合颗粒(T2)、生物炭颗粒(T3)、过氧化钙颗粒(T4)，其中土壤酚酸胁迫浓度为140μg/g。在番茄移栽后30和120天，测定了番茄生长和生理指标。在收获期，调查果实产量与品质，分析根际土壤理化性状，并使用高通量测序技术解析番茄根际土壤中细菌和真菌的群落结构特征。【结果】施用复合颗粒(T2处理)能够增强番茄根系活力，促进植株生长发育；单株番茄果实产量、单果重和糖酸比分别比T1处理增加了13.8%、20.1%和52.6%。与T1处理相比，T2处理番茄收获期根际土壤中残余总酚酸含量下降了44.6%，电导体(EC)值下降17.7%,pH提高0.77个单位，有机质含量增加77.4%。复合颗粒处理能够改善酚酸胁迫下番茄根际土壤微生物群落结构，使细菌多样性提高，真菌多样性降低，并有效恢复微生物群落...
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| 关 键 词： | 番茄 连作障碍 生物炭 过氧化钙 酚酸胁迫 土壤微生物 |
| 收稿时间： | 2022-06-07 |
Regulatory effects of biochar-calcium peroxide composite particles on tomato growth and rhizosphere microbial ecology under phenolic acid stress |
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| TU Yu-ting,PENG Zhi-ping,XU Yang-gui,LI Zhu-xian,HUANG Ji-chuan. Regulatory effects of biochar-calcium peroxide composite particles on tomato growth and rhizosphere microbial ecology under phenolic acid stress[J]. Plant Nutrition and Fertilizer Science, 2022, 28(10): 1914-1927. DOI: 10.11674/zwyf.2022292 |
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| Authors: | TU Yu-ting PENG Zhi-ping XU Yang-gui LI Zhu-xian HUANG Ji-chuan |
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| Affiliation: | Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences / Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation / Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture and Rural Affairs, Guangzhou, Guangdong 510640, China |
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| Abstract: | 【Objectives】 Phenolic acid stress is one of the primary factors limiting continuous cropping of tomato. The impact and mechanism of biochar-calcium peroxide composite particles as soil conditioners in relieving tomato phenolic acid stress were studied. 【Methods】 Pot experiments were carried out with ‘millennium cherry’ tomatoes and latosolic red soil. There were 5 treatments in total, including conventional cultivation (CK), phenolic acid stress treatment (T1), and exogenous phenolic acid together with biochar-calcium peroxide composite particles (T2), biochar particles (T3), and calcium peroxide particles (T4), respectively. The phenolic acid stress content in soil was 140 μg/g. The tomato growth and physiological indicators were examined at 30 and 120 days after transplanting tomato. At the harvest period, fruit production and quality, and rhizosphere soil physicochemical parameters were determined. High-throughput sequencing was employed to investigate the microbial diversity and community structure of in rhizosphere soil of tomato . 【Results】 Compared with T1, T2 treatment enhanced tomato root vigor and promoted plant growth, thus increased the yield, fruit weight and sugar-acid ratio of tomato by 13.8%, 20.1%, and 52.6%, respectively; decreased the total residual phenolic acid content and electrical conductivity (EC) in rhizosphere soil by 44.6% and 17.7%, increased pH by 0.77 units, and enhanced organic matter content by 77.4%. T2 treatment also enriched the microbial community structure, increased bacterial community diversity, reduced fungal community diversity, and effectively restored microbial community balance. According to the Spearman correlation heatmap and redundancy analysis, tomato yield and fruit sugar-acid ratio were negatively related with soil residual total phenolic acid and EC, but positively correlated with soil pH and organic matter content. Other than the reduced soil phenolic acids and EC as well as increased soil pH and organic matter content, the biochar-calcium peroxide composite soil conditioner selectively promoted the relative abundance of Bacteroidetes and Chytridiomycota, which are positively correlated with tomato yield and quality, and suppressed the relative abundance of Acidobacteria and Ascomycota, which are negatively correlated with tomato yield and quality. 【Conclusions】 The key environmental parameters driving the fluctuation of tomato rhizospheric microbial community are the residual total phenolic acid in soil, pH, organic matter content, and EC, which are all strongly related with tomato yield and quality. Through the dual regulation of soil physicochemical properties and microbial communities, biochar-calcium peroxide composite particles improves the micro-ecological environment of tomato rhizosphere, thereby effectively alleviating the allelopathic stress impact of phenolic acids on tomato growth and promoting fruit yield and quality. |
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