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放牧对松嫩草地羊草群落及土壤微生物群落碳源利用的影响
引用本文:张建峰, 庞思娜, 曲同宝. 放牧对松嫩草地羊草群落及土壤微生物群落碳源利用的影响[J]. 草业科学, 2014, 8(8): 1430-1436. DOI: 10.11829/j.issn.1001-0629.2013-0586
作者姓名:张建峰  庞思娜  曲同宝
作者单位:1.吉林农业大学生命科学学院, 吉林 长春 130118;2.吉林农业大学园艺学院, 吉林 长春 130118
摘    要:采用Biolog-ECO微平板检测法, 研究了短期放牧时3种放牧强度(不放牧、轻牧和重牧)下羊草(Leymus chinensis)群落特征变化、土壤理化性质和羊草群落根际土壤微生物群落碳源利用变化。结果表明, 轻牧有利于牧草繁殖;不同放牧强度会改变土壤微生物群落碳源利用情况, 土壤微生物代谢活性为轻牧最高, 不放牧最低;该羊草样地中利用多聚物的微生物群落占优势;多样性指数方面, 不放牧最高, 轻牧和重牧相近;主成分分析表明, 轻度放牧土壤中主要为利用碳水化合物类碳源的微生物, 重度放牧土壤中主要为利用氨基酸和多聚物类的微生物, 不放牧土壤微生物可利用碳源较少;理化性质对土壤微生物影响也较大。

关 键 词:Biolog法  土壤微生物  碳源利用
收稿时间:2013-10-18

Effect of grazing intensity on carbon source utilization of soil microorganism in rhizosphere of Leymus chinensis community in Songnen Grassland
Jian-feng ZHANG, Si-na PANG, Tong-bao QU. Effect of grazing intensity on carbon source utilization of soil microorganism in rhizosphere of Leymus chinensis community in Songnen Grassland[J]. Pratacultural Science, 2014, 8(8): 1430-1436. DOI: 10.11829/j.issn.1001-0629.2013-0586
Authors:Jian-feng ZHANG  Si-na PANG  Tong-bao QU
Affiliation:1.College of Life Science, Jilin Agricultural University, Changchun 130118, China;2.College of Horticulture, Jilin Agricultural University, Changchun 130118, China
Abstract:The Biolog-ECO microplate method was employed to study vegetation characteristics and carbon source utilization of soil microorganism community in rhizosphere of Leymus chinensis under three grazing intensity (without grazing, light grazing and heavy grazing) in short period. The results showed that there were difference in the carbon source utilization of soil microorganism community in rhizosphere of L.chinensis under three grazing intensities. Light grazing can promote the growth of herbage and had the highest metabolic activity. The treatment of without grazing had the lowest metabolic activity and more microorganisms using polymers. In the matter of diversity index, the treatment of without grazing had the highest diversity index which were lower and the treatments of both light grazing and heavy grazing were similar. Principle component analysis showed that the principal carbon source of soil microorganism of light grazing and heavy grazing treatments were carbohydrate, amino acid and polymer, respectively. There was less carbon source of soil microorganism for without grazing treatment. The soil physicochemical property had important impacts on soil microorganism under different grazing intensities.
Keywords:Biolog  soil microorganism  carbon source utilization
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