| 软枣猕猴桃受精后子房蛋白表达的2D-DIGE分析 |
| |
| 引用本文： | 齐秀娟,方金豹,陈锦永,顾红,张绍铃. 软枣猕猴桃受精后子房蛋白表达的2D-DIGE分析[J]. 中国农业科学, 2012, 45(17): 3646-3652. DOI: 10.3864/j.issn.0578-1752.2012.17.023 |
| |
| 作者姓名： | 齐秀娟 方金豹 陈锦永 顾红 张绍铃 |
| |
| 作者单位： | 1.南京农业大学梨工程技术研究中心，南京 2100952.中国农业科学院郑州果树研究所/果树生长发育与品质控制重点开放实验室，郑州 450009 |
| |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项基金(0032010032);郑州市重点科技攻关项目(121PPTGG466) |
| |
| 摘 要： | 【目的】了解软枣猕猴桃(Actinidia arguta)受精前和受精后（授粉前和授粉后120 h）子房蛋白质组的变化，为阐明猕猴桃属植物双受精的分子机制提供依据。【方法】应用石蜡切片技术、差异凝胶电泳（DIGE）、质谱（MALDI－TOF/TOF）和生物信息学技术，对其授粉前及授粉后120 h的子房形态及蛋白质组变化进行分析。【结果】授粉后120 h，子房中的胚囊已经完成了双受精；用DeCyder V 6.5软件分析，在授粉前、授粉后120 h的子房中共检测到约1 500个蛋白质点，其中55个有差异表达；质谱鉴定了差异2.0倍以上的蛋白质点13个，其中8个获得理想结果，分别属于6种蛋白质；在6种差异蛋白质中，其中5种在授粉后子房中表达上调，只有1种表达下调。【结论】根据软枣猕猴桃受精前后子房差异蛋白表达谱比较，获得了几种与双受精过程相关的蛋白质分子。
|
| 关 键 词： | 猕猴桃 受精 子房 蛋白质组 |
| 收稿时间： | 2012-03-01 |
Differential Protein Analysis of Chinese Gooseberry Ovary After Fertilization by a 2D-DIGE Approach |
| |
| QI Xiu-juan,FANG Jin-bao,CHEN Jin-yong,GU Hong,ZHANG Shao-ling. Differential Protein Analysis of Chinese Gooseberry Ovary After Fertilization by a 2D-DIGE Approach[J]. Scientia Agricultura Sinica, 2012, 45(17): 3646-3652. DOI: 10.3864/j.issn.0578-1752.2012.17.023 |
| |
| Authors: | QI Xiu-juan FANG Jin-bao CHEN Jin-yong GU Hong ZHANG Shao-ling |
| |
| Affiliation: | 1(1Pear Engineering Research Centre,Nanjing Agricultural University,Nanjing 210095;2Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory for Fruit Tree Growth,Development and Quality Control,Zhengzhou 450009) |
| |
| Abstract: | 【Objective】The changes of Chinese gooseberry (Actinidia arguta) ovary proteins and morphology before and 120 h after pollination were analyzed to provide valuable evidence for further elucidating the molecular mechanism of double fertilization.【Method】Fluorescence paraffin technique,differential in-gel electrophoresis (DIGE), Matrix-assisted laser-desorption/ionization Time of ?ight/Time of ?ight (MALDI-TOF/TOF) and bioinformatic technology were used in the experiment.【Result】The embryo sac in the ovary completed its double fertilization 120 h after pollination. About 1 500 protein points were detected by DeCyder 6.5 (Amersham Bioscience), and 55 proteins were differentially expressed at ovary before and 120 h after pollination. Thirteen protein spots (differential ratio＞2.0) were identified with MALDI-TOF/TOF, of these proteins,8 belong to 6 kinds of proteins. Five of the six proteins are up-regulated while one is down-regulated.【Conclusion】According to the expression differences of the proteins at ovary before and after fertilization, several proteins identified might be related to the process of the double fertilization of Chinese gooseberry. |
| |
| Keywords: | Chinese gooseberry fertilization ovary proteome |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
|
点击此处可从《中国农业科学》下载全文 |
