Post‐ovulatory aging of mouse oocytes in vivo and in vitro: Effects of caffeine on exocytosis and translocation of cortical granules |
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| Authors: | Jie Zheng Xun‐Qiang Yin Wei Ge Gui‐Fang He Wei‐Ping Qian Jun‐Yu Ma Wei Shen Shen Yin Qing‐Yuan Sun |
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| Affiliation: | 1. Key Laboratory of Animal Reproduction and Germplasm Enhancement in Universities of Shandong, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, Shandong, China;2. Institute of Reproductive Sciences, Qingdao Agricultural University, Qingdao, China;3. College of Life Science, Qingdao Agricultural University, Qingdao, China;4. Department of Reproductive Medicine;5. Peking University Shenzhen Hospital, Medical Center of Peking University, Shenzhen, Guangdong, China;6. State Key Laboratory of Reproductive Biology;7. Institute of Zoology, Chinese Academy of Sciences, Beijing, China |
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| Abstract: | The developmental potential of post‐ovulatory oocytes decreases with aging in vivo and in vitro. In this study, we aimed to investigate the effects of a potent antioxidant caffeine on cortical granules (CGs) distribution in mouse oocytes aging in vivo and in vitro. We found that in vivo administration of 150 mg/kg caffeine caused ovulation of some morphologically abnormal oocytes showing premature exocytosis or congregation of CGs, but significantly decreased abnormal distribution of CGs in oocytes aging for 6 h, 12 h and 18 h in vivo compared to those without caffeine treatment. Unexpectedly, supplementation of oocyte culture medium with 10 mmol/L caffeine accelerated CGs release of oocytes and the normal CG distribution rate dramatically decreased from 6 h in oocytes aging in vitro. It appeared that oocytes showed a high degree of abnormal CG distribution by aging for 18 h, and caffeine might delay oocyte CG exocytosis in vivo, but accelerates CG exocytosis in vitro. Our findings may have implications for improving assisted reproduction technologies. |
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| Keywords: | aging oocytes caffeine cortical granules |
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