| 寄主对云斑白条天牛幼虫取食行为及其体内酶活性的影响 |
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| 引用本文： | 吴广, 徐蕾, 李贝娜, 等. 寄主对云斑白条天牛幼虫取食行为及其体内酶活性的影响[J]. 西南林业大学学报（自然科学）, 2021, 41(3): 107–112.doi:10.11929/j.swfu.202004068 |
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| 作者姓名： | 吴广 徐蕾 李贝娜 彭思颖 郭春晖 杨振德 |
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| 作者单位： | 广西大学林学院，广西 南宁 530004 |
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| 摘 要： | 以核桃种群和桉树种群的云斑白条天牛幼虫为研究对象，通过人工更换新寄主饲养的方法研究云斑白条天牛幼虫对核桃和桉树的取食选择行为，采用分光光度计法测定更换新寄主前后幼虫体内消化酶、解毒酶和保护酶活性变化。明确不同寄主食物对云斑白条天牛取食行为及其体内酶活性的影响，探讨云斑白条天牛幼虫对寄主的适应机制。结果表明：云斑白条天牛幼虫偏好取食原寄主，但强迫幼虫取食新寄主能降低幼虫对原寄主取食的趋向率。不同寄主对云斑白条天牛幼虫体内消化酶、解毒酶和保护酶活性均有显著影响。其中，更换新寄主后幼虫体内的乙酰胆碱酯酶显著升高，而类胰蛋白酶、纤维素Cx酶、多酚氧化酶、谷胱甘肽−S−转移酶、超氧化物歧化酶显著降低。说明云斑白条天牛幼虫对原寄主具有“记忆”效应，更换寄主对其体内酶活性产生明显影响，乙酰胆碱酯酶可能在适应新寄主中发挥重要作用。
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| 关 键 词： | 云斑白条天牛 核桃树 桉树 取食趋性 酶活性 |
| 收稿时间： | 2020-04-27 |
Effects of Different Hosts on Feeding Behaviors and Enzyme Activities of Larvae of Batocera lineolata |
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| Guang Wu, Lei Xu, Beina Li, Siying Peng, Chunhui Guo and Zhende Yang. Effects of Different Hosts on Feeding Behaviors and Enzyme Activities of Larvae of Batocera lineolata[J]. Journal of Southwest Forestry University, 2021, 41(3): 107-112.doi:10.11929/j.swfu.202004068 |
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| Authors: | Guang Wu Lei Xu Beina Li Siying Peng Chunhui Guo Zhende Yang |
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| Affiliation: | College of Forestry, Guangxi University, Nanning Guangxi 530004, China |
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| Abstract: | The larvae of Batocera lineolata fed with Juglans regia and Eucalyptus sp. were used to investigate the feeding choice behavior to J. regia and Eucalyptus sp. through the artificial replacement of new host, and measure the enzymatic activity changes of digestive enzyme, detoxification enzyme and protective enzyme by the spectrophotometer method before and after the replacement of new host. This study aimed to illustrate the effect of different host foods towards the feeding behaviors and internal enzymatic activity of B. lineolata, and further explore the host adaptative mechanism of B. lineolata. The result shows that the larvae of B. lineolata preferred feeding the original host, but forcing the larvae to feed the new host could decrease the tendency towards feeding the original host. Different hosts could significantly affect the internal enzymatic activities of digestive enzymes, detoxifying enzymes and protective enzymes in the larvae of B. lineolata. Activities of acetylcholinesterase (AchE) increased significantly, while tryptase (TPS), cellulose CX enzyme (EG), polyphenol oxidase (PPO), glutathione-S-transferase (GSTs) and superoxide dismutase (SOD) decreased markedly after the replacement of new host. In conclusion, the larva of B. lineolata has a memory effect to the original host, and the replacement of host can dramatically impact their internal enzymatic activity. Acetylcholinesterase may make a difference in their adaptation to the new host. |
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| Keywords: | Batocera lineolata Juglans regia Eucalyptus sp. feeding tendency enzymatic activity |
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