| 不同硼效率甘蓝型油菜品种悬浮细胞的硼钙营养效应 |
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| 引用本文： | 王火焰,王运华. 不同硼效率甘蓝型油菜品种悬浮细胞的硼钙营养效应[J]. 植物营养与肥料学报, 2002, 8(1): 100-104. DOI: 10.11674/zwyf.2002.0119 |
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| 作者姓名： | 王火焰 王运华 |
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| 作者单位： | 1.华中农业大学作物遗传改良国家重点实验室; |
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| 基金项目： | 国家自然科学基金项目(39770439)资助 |
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| 摘 要： | 采用细胞培养的方法 ,研究了培养基中硼钙营养水平变化对不同硼效率甘蓝型油菜品种悬浮细胞生长及硼、钙和镁含量的影响。结果表明 ,增加硼营养显著促进了悬浮细胞的生长 ,钙对悬浮细胞生长的影响因硼供应状况和品种而异。两品种间差异表现为 ,低硼的抑制效应、高硼的促进效应以及较高钙的抑制效应都是硼低效 (对缺硼敏感 )品种Bakow(原代号 9141,曾用代号 01)比硼高效 (对缺硼不敏感 )品种特早 16 (原代号 9118,曾用代号 03)明显。增硼显著降低悬浮细胞的钙含量 ;镁含量因增硼先上升而后下降。增钙对悬浮细胞中硼含量无显著影响 ,硼钙镁之间存在着复杂的关系。
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| 关 键 词： | 油菜 细胞培养 硼效率 硼钙关系 |
| 文章编号： | 1008-505X(2002)01-0100-05 |
| 修稿时间： | 2000-01-17 |
Effects of boron-calcium nutrition on suspension-cell of rape cultivars with different boron efficiency |
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| WANG Huo yan,WANG Yun hua. Effects of boron-calcium nutrition on suspension-cell of rape cultivars with different boron efficiency[J]. Plant Nutrition and Fertilizer Science, 2002, 8(1): 100-104. DOI: 10.11674/zwyf.2002.0119 |
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| Authors: | WANG Huo yan WANG Yun hua |
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| Affiliation: | 1.National Key Lab. of Crop Genetic Improv.;Huazhong Agric. Univ.;Wuhan 430070;China; |
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| Abstract: | Effects of boron calcium nutrition on B, Ca, Mg concentration together with growth of suspension cell of two rape (Brassica napus L.) cultivars were studied. The results showed that, elevation of boron concentration in culture medium promoted growth of cells, but the effects of changing calcium supply on growth of cells were associated with rape cultivar and boron level. The boron inefficient cultivar Bakow was more sensitive to boron deficiency and more adaptable to higher boron supply than boron efficient cultivar Tezao16. Comparatively higher supply of calcium in culture medium hindered growth of cells,but the optimum level of calcium for cell growth of Tezao16 was higher than Bakow. It implicated that differences between genotypes in response to boron efficiency expressed at cellular level. The difference of necessary amount of boron for plant metabolism proposed to be the main cause which lead to genetic difference of boron efficiency between two rape cultivars. Increasing boron supply in culture medium significantly reduced calcium concentration in cells, while magnesium concentration in cells fluctuated as boron supply increased. There is no pronounced effect of calcium supply on boron concentration in cells. These results implicated a comprehensive relationship among boron, calcium and magnesium in rape cells. |
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