| 16份薏苡种质苗期耐寒性鉴定 |
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| 引用本文： | 普凤雅,唐然,谷书杰,方子松,杨志清. 16份薏苡种质苗期耐寒性鉴定[J]. 中国农学通报, 2020, 36(22): 5-12. DOI: 10.11924/j.issn.1000-6850.casb20200200091 |
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| 作者姓名： | 普凤雅 唐然 谷书杰 方子松 杨志清 |
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| 作者单位： | 1.云南农业大学农学与生物技术学院,昆明 650201;2.云南农业大学云南省药用植物生物学重点实验室,昆明 650201;3.云南农业大学西南中药材种质创新与利用国家地方联合工程研究中心,昆明 650201 |
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| 基金项目： | 云南省科技计划项目“云南薏苡规范化种植关键技术研究与示范”(2014RE002) |
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| 摘 要： | 本研究旨在筛选出抗寒性较高的薏苡种质,为薏苡杂交育种和栽培提供重要依据。以16份薏苡种质为试验材料,在3℃低温胁迫下培养3天,测定与抗寒性相关的生理指标,并采用极点排序法进行耐寒性综合评价。结果表明：低温(3℃)胁迫下,叶片叶绿素含量和超氧化物歧化酶(SOD)活性下降,种质叶片电导率含量、丙二醛含量、可溶性糖含量、脯氨酸含量、可溶性蛋白含量不同程度上升;在低温和常温的处理下,这些生理指标的变化有明显的差异,说明其变化与薏苡抗寒性密切相关;通过极点排序法得到薏苡抗寒能力排序：‘云野-6’<‘YGL’<‘云野-10’<‘云野-8’<‘云野-12’<‘云野-1’<‘云野-4’<‘云野-3’<‘云野-5’<‘YLO’<‘YOL-12’<‘云野-7’<‘云野-9’<‘云野-11’<‘云野-2’<‘Y9-5’。16个供试种质中‘Y9-5’的抗寒能力最强,‘云野-2’次之,‘云野-6’最弱,可将‘Y9-5’和‘云野-2’作为薏苡抗寒杂交育种和栽培的良好材料。
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| 关 键 词： | 薏苡 耐寒性 生理指标 极点排序法 综合评价 |
| 收稿时间： | 2020-02-07 |
Identification of Cold Tolerance in 16 Coix Germplasm |
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| Pu Fengya,Tang Ran,Gu Shujie,Fang Zisong,Yang Zhiqing. Identification of Cold Tolerance in 16 Coix Germplasm[J]. Chinese Agricultural Science Bulletin, 2020, 36(22): 5-12. DOI: 10.11924/j.issn.1000-6850.casb20200200091 |
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| Authors: | Pu Fengya Tang Ran Gu Shujie Fang Zisong Yang Zhiqing |
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| Affiliation: | 1.College of Agronomy and biotechnology, Yunnan Agricultural University, Kunming 650201;2.The Key Laboratory of Medicinal Plant Biology of Yunnan Province, Yunnan Agricultural University, Kunming 650201;3.National & Local Joint Engineering Research Center on Germplasm Innovation & Utilization of Chinese Medicinal Materials in Southwestern China, Yunnan Agricultural University, Kunming 650201 |
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| Abstract: | The research aims to screen out the germplasm of Coix with high cold resistance, and provide a basis for hybrid breeding and cultivation of Coix. Taking 16 germplasms of Coix as test materials, we cultured the materials under low temperature stress at 3℃ for 3 days, measured the physiological indexes related to cold resistance, and comprehensively evaluated cold resistance by using a pole-sorting method. The results showed that under low temperature (3℃) stress, the chlorophyll content and SOD activity of leaves decreased, and the electrical conductivity content, malondialdehyde content, soluble sugar content, proline content, and soluble protein content of leaves of various germplasms increased to varying degrees. Under the treatment of low temperature and normal temperature, there were obvious differences in the change of these physiological indicators, indicating that the changes are closely related to the cold resistance of the Coix. The rank of the cold resistance of the Coix obtained by the pole-sorting method is: ‘Yunye-6’<‘YGL’<‘Yunye-10’<‘Yunye-8’<‘Yunye-12’<‘Yunye-1’<‘Yunye-4’<‘Yunye-3’<‘Yunye-5’<‘YLO’<‘YOL-12’<‘Yunye-7’<‘Yunye-9’<‘Yunye-11’<‘Yunye-2’<‘Y9-5’. Among the 16 germplasms, ‘Y9-5’ and ‘Yunye-2’could be used as cold resistance material for hybrid breeding and cultivation. |
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| Keywords: | Coix cold tolerance physiological indexes pole-sorting method comprehensive evaluation |
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