| 高能碳离子束辐射对玉米当代的生物学效应 |
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| 引用本文： | 李军华,蔡青,郭会君,周利斌,宋希云,郭新梅. 高能碳离子束辐射对玉米当代的生物学效应[J]. 核农学报, 2022, 36(4): 681-688. DOI: 10.11869/j.issn.100-8551.2022.04.0681 |
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| 作者姓名： | 李军华 蔡青 郭会君 周利斌 宋希云 郭新梅 |
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| 作者单位： | 青岛农业大学农学院/青岛市主要农作物种质创新与应用重点实验室,山东 青岛 266109;中国农业科学院作物科学研究所,北京 100081;中国科学院近代物理研究所,甘肃 兰州 730000 |
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| 基金项目： | 国家重点研发计划课题(2016YFD0102104)； |
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| 摘 要： | 为了研究高能碳离子诱变对玉米的影响,本研究利用高能碳离子处理昌7-2和PH6WC两个玉米自交系,分析当代植株的出苗率、株高、穗位高等表型,测定收获后的籽粒性状。结果表明,经高能碳离子辐射后,两个玉米自交系的出苗率显著降低,且在高剂量辐射下两个玉米自交系在2019年均有降低。植株表型分析结果发现,在2019年,昌7-2和PH6WC在40~100 Gy辐射下株高显著下降;在2020年,PH6WC在150 Gy辐射下穗位高显著下降;在2019年,昌7-2在60~100 Gy辐射下穗位高显著下降;在2019年,昌7-2在80~100 Gy辐射下叶夹角增大;在2019年,昌7-2在40、100 Gy辐射下叶长显著降低,PH6WC在100 Gy辐射下叶长和叶宽显著下降;在2019年,昌7-2在80 Gy辐射下叶宽显著增加,在20 Gy辐射下雄穗分枝显著增加,而100 Gy辐射下雄穗分枝显著降低。在籽粒性状中,高剂量(60~100 Gy)辐射下的粒厚显著增加,粒长降低但百粒重有所增加,昌7-2比PH6WC更为明显。由上述结果可知,不同玉米种质的不同性状对高能碳离子的诱变处理效应存在差异,40~60 Gy是昌7-2较为适宜的诱变剂量,而PH6WC较为适宜的剂量为60~80 Gy。本研究结果可为玉米种子的高能碳离子诱变剂量筛选提供一定的理论依据。
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| 关 键 词： | 高能碳离子束 玉米 当代 生物学效应 |
| 收稿时间： | 2021-04-21 |
Biological Effects of High Energy Carbon Ion Beam Irradiation on Contemporary Maize |
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| LI Junhua,CAI Qing,GUO Huijun,ZHOU Libin,SONG Xiyun,GUO Xinmei. Biological Effects of High Energy Carbon Ion Beam Irradiation on Contemporary Maize[J]. Acta Agriculturae Nucleatae Sinica, 2022, 36(4): 681-688. DOI: 10.11869/j.issn.100-8551.2022.04.0681 |
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| Authors: | LI Junhua CAI Qing GUO Huijun ZHOU Libin SONG Xiyun GUO Xinmei |
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| Affiliation: | 1Agricultural College of Qingdao Agricultural University/Qingdao Key Laboratory of Innovation and Application of Main Crop Germplasm,Qingdao, Shandong 2661092Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 1000813Institute of Modern Physics, Chinese Academy of Science, Lanzhou, Gansu 730000 |
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| Abstract: | In order to study the effect of high energy carbon ion on maize, two maize inbred lines Chang 7-2 and PH6WC were treated with high energy carbon ion. The seedling emergence rate, plant height, ear position and other phenotypes of modern plants were analyzed, and the grain characters after harvest were determined. The results showed that high energy carbon ion treatment had different effects on different maize materials, and the sensitivity of different characters to high energy carbon ion was varied. The emergence rate of two maize inbred lines decreased significantly after high energy carbon ion irradiation, and the survival rate was very low under high dose irradiation. The plant height of Chang 7-2 and PH6WC decreased significantly under 40~100 Gy irradiation, the ear height of PH6WC decreased significantly under 100 Gy irradiation, the ear height of Chang 7-2 decreased significantly under 60~100 Gy irradiation, the leaf angle of Chang 7-2 increased under 80~100 Gy irradiation, the leaf length of chang7-2 decreased significantly under 40~100 Gy irradiation, the leaf length and width of PH6WC decreased significantly under 100 Gy irradiation, and the leaf width of Chang 7-2 increased significantly under 80~100 Gy irradiation. In addition, tassel branch number of Chang 7-2 increased significantly under 20 Gy irradation, but decreased significantly at 100 Gy irradation. For the grain characters, the grain thickness increased significantly and the grain length decreased, but the 100 grain weight increased significantly. Therefore, 40~60 Gy is the most suitable mutagenic dose for Chang 7-2, while 60~80 Gy is the most suitable dose for PH6WC. The results of this study can provide a certain basis for high energy carbon ion mutation dose of maize seeds. |
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| Keywords: | high energy carbon ion beam maize contemporary biological effect |
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