| 小飞蓬多酚提取及其抗氧化性研究 |
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| 引用本文： | 贤景春,赖金辉,刘玉明. 小飞蓬多酚提取及其抗氧化性研究[J]. 南方农业学报, 2012, 43(11): 1749-1753. DOI: 10.3969/j:issn.2095-1191.2012.11.1749 |
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| 作者姓名： | 贤景春 赖金辉 刘玉明 |
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| 作者单位： | 泉州师范学院化学与生命科学学院,福建泉州,362000内蒙古民族大学物理与电子信息学院,内蒙古通辽,028043 |
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| 基金项目： | 泉州市科技支撑计划项目(2007N4) |
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| 摘 要： | [目的]确定小飞蓬多酚最佳提取工艺及其对羟基自由基(·OH)的清除效果,为充分开发利用小飞蓬的药用价值奠定基础.[方法]用乙醇浸提法提取小飞蓬多酚,采用单因素试验与正交试验优化乙醇浓度、浸提温度、浸提时间、料液比等提取工艺参数,并探讨小飞蓬多酚对·OH的清除作用.[结果]各提取因素对小飞蓬多酚提取效果的影响顺序为:乙醇浓度>料液比>浸提时间>浸提温度,最佳提取工艺参数为:乙醇浓度50%,浸提温度70℃,浸提时间60min,料液比1∶20.当小飞蓬多酚浓度为0.3 mg/mL时,对·OH的清除率可达35%.[结论]在最佳提取工艺条件下小飞蓬多酚的提取率可达12.751 mg/g,且小飞蓬多酚对·OH的清除效果良好.
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| 关 键 词： | 小飞蓬 多酚 提取工艺 羟基自由基 抗氧化性 |
Conyza canadensis L.Cronq.polyphenol extraction and its antioxidation activities |
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| XIAN Jing-chun,LAI Jin-hui,LIU Yu-ming. Conyza canadensis L.Cronq.polyphenol extraction and its antioxidation activities[J]. Journal of Southern Agriculture, 2012, 43(11): 1749-1753. DOI: 10.3969/j:issn.2095-1191.2012.11.1749 |
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| Authors: | XIAN Jing-chun LAI Jin-hui LIU Yu-ming |
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| Affiliation: | 1College of Chemistry and Life Science,Quanzhou Normal University,Quanzhou,Fujian 362000,China;2College of Physics and Electronic Information,Inner Mongolia University for Nationalities,Tongliao,Inner Mongolia 028043,China) |
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| Abstract: | 【Objective】This research determined the effects of Conyza canadensis polyphenol extraction process on the scavenging of hydroxyl radical （·OH） in order to improve the development of Conyza canadensis medical resources. 【Method】The Conyza canadensis polyphenol extracted through the ethanol extraction method was tested under the single factor test and the orthogonal test to optimize the following extraction process parameters: ethanol concentration， extraction temperature， extraction time duration， and solid-to-solution ratio. These parameters were then analyzed to explore the effects of Conyza canadensis polyphenol extraction on ·OH removal. 【Result】The effect order of the Conyza canadensis polyphenol extraction factors were arranged as follows: ethanol concentration>solid-to-solution ratio>extraction time duration>extraction temperature. The optimum extraction process parameters were 50% ethanol concentration， 70℃ extraction temperature， 60 min extraction duration， and 1∶20 solid-to-solution ratio. When the Conyza canadensis polyphenol concentration reached 0.3 mg/mL， the ·OH removal rate could increase up to 35%. 【Conclusion】Under the optimum extracting conditions， the Conyza canadensis polyphenol extraction rate could reach 12.751 mg/g； also， under this extraction rate， the removal effects of Conyza canadensis polyphenol on ·OH was excellent. |
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