| 检测马疱疹病毒4型抗体间接ELISA方法的建立及应用简 |
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| 引用本文： | 田志革,郭巍,赵世华,等. 检测马疱疹病毒4型抗体间接ELISA方法的建立及应用简[J]. 中国畜牧兽医, 2014, 41(1): 11-14 |
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| 作者姓名： | 田志革 郭巍 赵世华 等 |
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| 摘 要： |
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Establishment and Application of ELISA for Detection of Equine Herpesviruses Type 4 Antibodies |
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| TIAN Zhi-ge,GUO Wei,ZHAO Shi-hua,PAN Jia-liang,RAN Duo-liang,XIANG Wen-hua,QU Juan-juan. Establishment and Application of ELISA for Detection of Equine Herpesviruses Type 4 Antibodies[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(1): 11-14 |
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| Authors: | TIAN Zhi-ge GUO Wei ZHAO Shi-hua PAN Jia-liang RAN Duo-liang XIANG Wen-hua QU Juan-juan |
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| Abstract: | In order to establish an indirect ELISA method for detection of equine herpesviruses type 4 （EHV4） antibody, the glycoprotein G （gG） protein with specific epitope worked as a detection antigen. After optimizing conditions, the indirect ELISA method was developed successfully,specificity and repeatability tests were determined. The result was that the EHV4 gG only reacted with antibodies against EHV4;but not with antibodies against EHV1; both the intro-batch and inter-batch variation coefficiencies were lower than 10%.Concordance of the indirect ELISA relative to commercial EHV1/4 antibody kit was above 90%.The results indicated that the indirect ELISA method could be used for the detection and epidemiological surveys of EHV4 infection. |
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| Keywords: | detection equine herpesviruses type 4 glycoprotein G indirect ELISA |
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