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| 运输应激对驴血液指标的影响 | |
| 引用本文： | 高维平,姜桂苗,梁伟超,李俊桥,嵇传良,赵付伟,邓立新. 运输应激对驴血液指标的影响[J]. 中国畜牧兽医, 2019, 46(2): 373-379. DOI: 10.16431/j.cnki.1671-7236.2019.02.006 |
| 作者姓名： | 高维平 姜桂苗 梁伟超 李俊桥 嵇传良 赵付伟 邓立新 |
| 作者单位： | 1. 国家胶类中药工程技术研究中心, 东阿阿胶股份有限公司, 东阿 252201; 2. 河南农业大学牧医工程学院, 郑州 450002 |
| 基金项目： | 山东省农业良种工程项目（2017LZGC020） |
| 摘 要： | 本试验旨在研究长途运输应激对驴血液指标的影响。试验以12头体重(140.8±5.2)kg的德州驴为研究对象,运输距离约950km,发车地气温为-20℃,目的地气温为-10℃,历时21h,其中包括10次停车观察(≤20min)。分别在运输前、运输后0h和运输后3、5、7、9、15d采集颈静脉血15mL,进行血液激素指标、血常规指标和血液生化指标检测,并分别记录运输前、运输后0h和运输后10、20、30d体重。结果显示,与运输前相比,落地后0h血清中促肾上腺皮质激素(ACTH)、热休克蛋白90(HSP90)和皮质醇(cortisol)浓度均显著升高(P<0.05),落地后3、5、7、9、15d内逐渐降低,ACTH和HSP90基本恢复至运输前水平;中性粒细胞总数(Neu)、红细胞总数(RBC)和血红蛋白浓度(HGB)均较运输前显著上升(P<0.05),白细胞总数(WBC)有所上升但与运输前差异不显著(P>0.05),淋巴细胞总数(LYM)较运输前显著下降(P<0.05);血清总蛋白(TP)、血清白蛋白(ALB)、肌酐(CREA)、乳酸脱氢酶(LDH)、运输前后均未发生显著变化(P>0.05),谷草转氨酶(AST)、肌酸激酶(CK)、尿素(UREA)均较运输前显著上升(P<0.05);体重较运输前显著下降(P<0.05),运输后10d内体重已恢复至运输前水平。上述结果表明,长途运输可诱发驴的应激反应、肝脏损伤、机体代谢水平升高等,但免疫系统未受到明显损害,在落地后15d内机体逐渐从应激反应中恢复至运输前状态。 |
| 关 键 词： | 驴 运输 应激 血液指标 |
| 收稿时间： | 2018-07-16 |
Effect of Transport Stress on Haemotological Indices of Donkey |
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| GAO Weiping,JIANG Guimiao,LIANG Weichao,LI Junqiao,JI Chuanliang,ZHAO Fuwei,DENG Lixin. Effect of Transport Stress on Haemotological Indices of Donkey[J]. China Animal Husbandry & Veterinary Medicine, 2019, 46(2): 373-379. DOI: 10.16431/j.cnki.1671-7236.2019.02.006 | |
| Authors: | GAO Weiping JIANG Guimiao LIANG Weichao LI Junqiao JI Chuanliang ZHAO Fuwei DENG Lixin |
| Affiliation: | 1. National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co., Ltd., Donge 252201, China; 2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China |
| Abstract: | This study was designed to investigate the effects of long-distance transport stress on blood parameters of donkey.The study was conducted with 12 heads of Dezhou donkeys with weight of (140.8±5.2) kg,the transportation distance was about 950 km,the departure temperature was -20℃,and the destination temperature was -10℃,which lasted for 21 h,including 10 parking observations (≤ 20 min).15 mL of jugular venous blood was collected before loading,0 h after transportation and 3,5,7,9 and 15 d after transportation,and blood hormone indexes,blood routine indexes and blood biochemical indexes were detected,and the weights before transport,0 h after transport and 10,20 and 30 d after transport were recorded.The results showed that the concentrations of adrenocorticotropic hormone (ACTH),heat shock protein 90 (HSP90) and cortisol in the serum at 0 h after landing were significantly higher than those before transportation (P<0.05),and gradually decreased within 3,5,7,9 and 15 d after transportation,and ACTH and HSP90 recovered to pre-carriage levels.The number of neutrophils (Neu),the total number of red blood cells (RBC) and the hemoglobin concentration (HGB) increased significantly after 3,5,7,9 and 15 d (P<0.05),the total number of white blood cells (WBC) increased but not significantly different from that before transportation (P>0.05),the total number of lymphocyte (LYM) was significantly lower than before transport (P<0.05).There were no significant changes in serum total protein (TP),serum albumin (ALB),creatinine (CREA) and lactate dehydrogenase (LDH) before and after transport (P>0.05).Aspartate aminotransferase (AST),creatine kinase (CK) and urea (UREA) were significantly higher than before transport (P<0.05).The body weight decreased significantly (P<0.05),and recovered to the pre-transport level within 10 d after transportation.The above results indicated that long-distance transport induced stress response to sputum,liver damage and elevated levels of metabolism,but the immune system was not significantly damaged.Within 15 d after landing,the body gradually recovered from the stress response to the pre-shipment state. |
| Keywords: | donkey transport stress haemotological indices |
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