| 壳聚糖酶解产物抑制真菌活性研究 |
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| 引用本文： | 王军华,赵双枝,陈相艳,张彦昊,辛雪,张翔,陈蕾蕾. 壳聚糖酶解产物抑制真菌活性研究[J]. 核农学报, 2021, 35(3): 660-666. DOI: 10.11869/j.issn.100-8551.2021.03.0660 |
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| 作者姓名： | 王军华 赵双枝 陈相艳 张彦昊 辛雪 张翔 陈蕾蕾 |
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| 作者单位： | 山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室,山东济南250100;山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室,山东济南250100;山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室,山东济南250100;山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室,山东济南250100;山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室,山东济南250100;山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室,山东济南250100;山东省农业科学院农产品研究所/山东省农产品精深加工技术重点实验室/农业农村部新食品资源加工重点实验室,山东济南250100 |
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| 基金项目： | 国家重点研发计划项目(2017YFC1601400),山东省农业科学院青年科研基金(2016YQN49),山东省外专双百计划项目(WST2017004),山东省重点研发计划(2017GSF216007) |
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| 摘 要： | 为探究并提高壳聚糖抑制真菌的活性,本研究利用蜡状芽孢杆菌ncps116发酵所制备的壳聚糖酶来酶解壳聚糖,测定不同酶解时间壳聚糖产物的抑菌活性,并监测酶解产物中还原糖的含量;之后选取抑菌活性提高最显著的壳聚糖酶解产物为研究对象,测定最低抑菌活性和最适pH,并使用电子显微镜观察酶解产物对病原真菌菌丝和孢子萌发的抑制作用.结...
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| 关 键 词： | 壳聚糖酶解 抗真菌活性 抑菌机制 蜡状芽孢杆菌 |
| 收稿时间： | 2019-09-20 |
Antifungal Activities of Chitosan Hydrolysates |
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| WANG Junhua,ZHAO Shuangzhi,CHEN Xiangyan,ZHANG Yanhao,XIN Xue,ZHANG Xiang,CHEN Leilei. Antifungal Activities of Chitosan Hydrolysates[J]. Acta Agriculturae Nucleatae Sinica, 2021, 35(3): 660-666. DOI: 10.11869/j.issn.100-8551.2021.03.0660 |
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| Authors: | WANG Junhua ZHAO Shuangzhi CHEN Xiangyan ZHANG Yanhao XIN Xue ZHANG Xiang CHEN Leilei |
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| Affiliation: | Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing Technology of Shandong Province/Key Laboratory of Novel Food Resources Processing,Ministry of Agriculture and Rural Affairs, Jinan, Shandong 250100 |
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| Abstract: | In previous studies, the inhibiting activities of the commercially available chitosan on the fungus was significantly improved after hydrolysis. For the development of the fungicidal activities, chitosan hydrolysates was prepared using chitosanase from fermentation of Bacillus cereus ncps116 in this study. The fungicidal activities and reducing sugar content were analyzed in the process of enzymatic hydrolysis with time going. The most active hydrolysate was chosen to be tested the minimum antibacterial concentration and the optimum pH. Inhibition to the hyphae and spore was investigated under electron microscopy. The results showed that the activity was increased by the hydrolysis. It increased firstly and then decreased with time going within 24 h, which was related to sugar content. The hydrolysates with too low (≤44.24 μg·mL-1) or too high (≥1 900 μg·mL-1) reducing sugar content, which mean that hydrolysis was insufficient or complete, showed bad activities with he diameters of the inhibtion zone around 13 and 9 mm, respectively. The hydrolysate at 6 h showed the most significant fungicidal activities, which affected the growth of the tested pathogenic fungi, including Verticillium dahliae, Physalospora piricola, Fusarium oxysporum f.sp. niveum, with a 4 to 8-fold improvement at minimum fungicidal concentration around 0.125%~0.250%. The concentration of reducing sugar was tested to be 383.34 μg·mL-1. Besides, the hydrolysates exhibited the most high antifungal bioactivity at pH 4.0~4.7. Furthermore, the hydrolysates could cause disrupt and abnormal growth of fungal hyphae, such as folds and vesicles. And the inhibition to the spore and mycelial growth was irreversible for a long time. In a word, the hydrolysis could significantly improve the fungicidal activities of chitosan, which provided a research basis for the further application on chitosan in agricuture. |
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| Keywords: | chitosan hydrolysates fungicidal activities mechanism Bacillus cereus |
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