| 高粱病程相关基因非表达子1(NPR1)基因家族鉴定及胁迫应答分析 |
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| 引用本文： | 杜巧丽,方远鹏,蒋君梅,孙涛,任明见,谢鑫. 高粱病程相关基因非表达子1(NPR1)基因家族鉴定及胁迫应答分析[J]. 核农学报, 2021, 35(5): 1074-1083. DOI: 10.11869/j.issn.100-8551.2021.05.1074 |
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| 作者姓名： | 杜巧丽 方远鹏 蒋君梅 孙涛 任明见 谢鑫 |
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| 作者单位： | 贵州大学农学院农业微生物特色重点实验室,贵州贵阳 550025;重庆海关技术中心,重庆 400020;贵州大学农学院农业微生物特色重点实验室,贵州贵阳 550025;国家小麦改良中心贵州分中心,贵州贵阳 550025 |
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| 基金项目： | 国家自然科学基金(32060614、31801691),贵州省科技计划(黔科合支撑[2019]2408号),贵州省高层次留学人才创新创业择优资助项目[(2018)02号] |
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| 摘 要： | 非表达因子基因(NPR1)是植物系统获得抗性的激活子,也是植物响应病原菌侵染过程中的核心因子之一,在植物抗病性方面发挥着非常重要的作用.为明确高粱NPR1基因家族成员及SbNPR1的表达特性,本研究通过生物信息学及实时荧光定量PCR(RT-qPCR)方法,对高粱NPR1基因家族进行鉴定和表达分析.结果表明,共鉴定到5个...
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| 关 键 词： | 高粱 NPR1 基因表达分析 胁迫应答 PAMPs |
| 收稿时间： | 2020-01-31 |
Identification of Sorghum Non-Expressor of Pathogenesis Related Genes 1 Gene Family and Analysis of Their Expression Under Different Stresses |
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| DU Qiaoli,FANG Yuanpeng,JIANG Junmei,SUN Tao,REN Mingjian,XIE Xin. Identification of Sorghum Non-Expressor of Pathogenesis Related Genes 1 Gene Family and Analysis of Their Expression Under Different Stresses[J]. Acta Agriculturae Nucleatae Sinica, 2021, 35(5): 1074-1083. DOI: 10.11869/j.issn.100-8551.2021.05.1074 |
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| Authors: | DU Qiaoli FANG Yuanpeng JIANG Junmei SUN Tao REN Mingjian XIE Xin |
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| Affiliation: | 1Key Laboratory of Agricultural Microbiology, College of Agricultural, Guizhou University, Guiyang, Guizhou 550025;2Guizhou Branch of National Wheat Improvement Center, Guiyang, Guizhou 550025;3Technical Center of Chongqing Customs, Chongqing 400020 |
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| Abstract: | NPR1 (non-expressor of PR genes 1) gene is an activator of plant system acquired resistance, and also one of the most important core factors in plant response to pathogen infection, it plays an important role in plant disease resistance. In order to classify the NPR1 gene family and verify the expression pattern of SbNPR1, bioinformatics and real-time fluorescence quantitative analysis (RT-qPCR) were used to analyze the NPR1 gene family and the expression of SbNPR1 expression respectively. The results showed that 5 NPR1 genes, SbNPR1~SbNPR5, were identified in sorghum and the length of amino acid sequence is 480~621 aa, the theoretical molecular weight is between 50.496 81~67.648 06 kDa, theory of isoelectric point is 5.64~6.11. Phylogenetic analysis showed that SbNPR1 was closely related to Sh253P03. Genetic structure analysis showed that the number of exons and introns varied little among members of the family. RT-qPCR analysis showed that the expression of SbNPR1 gene had tissue specificity in sorghum plant. The expression of SbNPR1 was inhibited and induced respectively by the treatment of hormone cyclophosphamide (GR24, 1 μmol·L-1) and salicylic acid (SA, 1 mmol·L-1). The expression of SbNPR1 showed a trend of first increasing and then decreasing treated by PEG6000 (20%) and NaCl (250 mmol·L-1), the relative expression levels were reached the maximum at 0.5 h, and then decreased. However, after treatment with Mannitol (D-Mannitol, 300 mmol·L-1), the expression level of SbNPR1 gene decreased significantly after 3 h. Sorghum bicolor is treated with pathogen-associated molecular pattern receptors (PAMPs) flg22 (100 nmol·L-1) and Chitin (8 nmol·L-1). The expression of SbNPR1 was induced by flg22 and reached to the highest at 12 h after treatment, while its expression was inhibited by Chitin treatment. This study provides a basis for further exploring the role of NPR1 family in regulating sorghum resistance, signal transduction, plant hormones and stress regulation. |
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| Keywords: | Sorghum NPR1 gene expression analysis stress response PAMPs |
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