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转基因(Bn-csRRM2)高产棉花对土壤速效养分和酶活性的影响
引用本文:赵云丽,赵建宁,李刚,修伟明,曹璇,王慧,马艳,多立安,杨殿林. 转基因(Bn-csRRM2)高产棉花对土壤速效养分和酶活性的影响[J]. 棉花学报, 2015, 27(2): 143-148. DOI: 1002-7807(2015)02-0143-06
作者姓名:赵云丽  赵建宁  李刚  修伟明  曹璇  王慧  马艳  多立安  杨殿林
作者单位:1. 天津师范大学生命科学学院,天津300387;农业部环境保护科研监测所,农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室,天津300191
2. 农业部环境保护科研监测所,农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室,天津300191
3. 天津农学院农学与资源环境学院,天津,300384
4. 中国农业科学院棉花研究所,河南安阳,455000
5. 天津师范大学生命科学学院,天津,300387
基金项目:转基因生物新品种培育重大专项(2013ZX08011-002);国家自然科学基金(31200424);中央级公益性科研院所基本科研业务费专项资金(农业部环境保护科研监测所) 和农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室开放基金项目
摘    要:在田间试验条件下,以转基因(Bn-cs RRM2)高产棉及其亲本常规棉中棉所12为研究对象,比较分析2种棉花在不同生育期(苗期、蕾期、花铃期和吐絮期)对土壤速效养分和酶活性的影响。结果表明,随生育期推进,转基因高产棉土壤铵态氮和硝态氮含量变化趋势与常规棉一致,土壤速效磷含量变化趋势与常规棉不同。转基因高产棉土壤速效磷含量在蕾期、花铃期和吐絮期与常规棉无显著差异;铵态氮含量在苗期、蕾期和花铃期与常规棉无显著差异;硝态氮含量在吐絮期显著高于常规棉,其余3个时期显著低于常规棉。随生育期推进,转基因高产棉土壤脲酶和过氧化氢酶活性变化趋势与常规棉一致,碱性磷酸酶活性变化趋势与常规棉略有不同。转基因高产棉土壤脲酶活性在苗期、蕾期和吐絮期显著高于常规棉,在花铃期无显著差异;过氧化氢酶活性在苗期和吐絮期与常规棉无显著差异;碱性磷酸酶活性只在吐絮期与常规棉无显著差异。表明,土壤养分和酶活性受转基因高产棉的影响较小,其变化主要受到生育期的影响。

关 键 词:转基因  棉花  速效养分  土壤酶活性  高产
收稿时间:2014-08-28;

Changes in Available Nutrients and Enzyme Activities in Soil of Transgenic(Bn-csRRM2) High-yield Cotton
Zhao Yunli,Zhao Jianning,Li Gang,Xiu Weiming,Cao Xuan,Wang Hui,Ma Yan,Duo Li'an,Yang Dianlin. Changes in Available Nutrients and Enzyme Activities in Soil of Transgenic(Bn-csRRM2) High-yield Cotton[J]. Cotton Science, 2015, 27(2): 143-148. DOI: 1002-7807(2015)02-0143-06
Authors:Zhao Yunli  Zhao Jianning  Li Gang  Xiu Weiming  Cao Xuan  Wang Hui  Ma Yan  Duo Li'an  Yang Dianlin
Abstract:This study monitored the changes in available nutrients and enzyme activities in the soils of transgenic(Bn-csRRM2) high-yield cotton and its parental isoline CRI 12 in different growth stages(seeding, squaring, flower and boll, and boll-opening stage) under field conditions. Results showed that ammonium nitrogen and nitrate nitrogen contents were not significantly different over time between cultivars, but the available phosphorus content was different over time between cultivars. The available phosphorus content was not significantly different between cultivars in squaring, flower and boll, and boll-opening stages. The ammonium nitrogen content was not significantly different between cultivars in seeding, squaring and flower and boll stages. The nitrate nitrogen content of transgenic high-yield cotton was significantly higher than that of conventional cotton in boll-opening stage and lower than conventional cotton in the other three growth stages. The urease and catalase activities were not significantly different between cultivars over time, but the alkaline phosphatase activity of transgenic high-yield cotton was slightly different from that of conventional cotton over time. Urease activity of transgenic high-yield cotton was significantly higher than that of conventional cotton in seeding, squaring, and boll-opening stages, but was not significantly different from that of conventional cotton in the flower and boll stage. There was no significant difference in catalase activity between cultivars in the seeding and boll-opening stages. Boll-opening stage was the only stage in which the alkaline phosphatase activity was not significantly different between cultivars. Our results indicated that the available nutrient contents and enzyme activities of soil were less affected by transgenic high-yield cotton, but mainly affected by the growth stage.
Keywords:cotton  available nutrients  soil enzyme activity  high-yield
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