| 大豆重组自交系群体NJRIKY遗传图谱的加密及其应用效果 |
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| 引用本文： | 周斌,邢邯,陈受宜,盖钧镒. 大豆重组自交系群体NJRIKY遗传图谱的加密及其应用效果[J]. 作物学报, 2010, 36(1): 36-46. DOI: 10.3724/SP.J.1006.2010.00036 |
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| 作者姓名： | 周斌 邢邯 陈受宜 盖钧镒 |
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| 作者单位： | 1南京农业大学大豆研究所/国家大豆改良中心/作物遗传生理重点实验室，南京210095；2中国科学院遗传与发育生物学研究所植物基因组学国家重点实验室，北京100101 |
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| 基金项目： | 本研究由国家重点基础研究发展计划（973计划）项目（2006CB101708,2009CB118404），国家高新技术研究发展计划（863计划）项目（2006AA100104），教育部高等学校学科创新引智计划项目（B08025），农业部公益性行业专项（200803060）资助。 |
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| 摘 要： | 作物基因组研究,包括基因或数量性状位点(QTL)定位、图位克隆以及物理图谱构建等,首先必须建立具有丰富标记信息的高密度遗传连锁图谱。由科丰1号和南农1138-2杂交组合衍生的重组自交系群体NJRIKY已经构建了4张大豆遗传连锁图谱,但由于遗传信息和标记数目不够充分,在基因和QTL作图时仍然存在精确度和准确度问题。为增加NJRIKY图谱密度,本研究在967对SSR引物中获得了401个多态性SSR标记。结合其他分子数据,使用作图软件Mapmaker/Exp3.0b,获得一张含有553个遗传标记,25个连锁群,总长2071.6cM,平均图距3.70cM的新遗传连锁图谱,其中SSR标记316个,RFLP标记197个,EST标记39个,形态标记1个。连锁群上大于20cM的标记间隔由原来42个减少到2个。原图谱的3个SMV抗性基因定位于D1b连锁群末端的开放区间上且仅与一个RFLP标记连锁,利用加密图谱对Rsc-3、Rsc-7、Rsc-9、Rsc-13、Rsa、Rn1和Rn3等7个SMV抗性基因重定位,全部位于D1b连锁群,与相邻分子标记距离均小于6cM,其中Rsc-9、Rn1、Rsa的距离小于1cM,Rsc-13与EST标记GMKF168a共分离。对本群体农艺性状进行QTL重定位,获得8个性状相关的42个主效QTL,其中20个QTL遗传贡献率大于10%,与原图谱比较,新定位的各QTL的标记区间明显缩短,与相邻标记的连锁更加紧密。
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| 关 键 词： | 大豆 遗传连锁图谱 SSR标记 QTL |
| 收稿时间： | 2009-05-08 |
| 修稿时间： | 2009-10-02 |
Density-Enhanced Genetic Linkage Map of RIL Population NJRIKY and Its Impacts on Mapping Genes and QTLs in Soybean |
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| ZHOU Bin,XING Han,CHEN Shou-Yi,GAI Jun-Yi. Density-Enhanced Genetic Linkage Map of RIL Population NJRIKY and Its Impacts on Mapping Genes and QTLs in Soybean[J]. Acta Agronomica Sinica, 2010, 36(1): 36-46. DOI: 10.3724/SP.J.1006.2010.00036 |
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| Authors: | ZHOU Bin XING Han CHEN Shou-Yi GAI Jun-Yi |
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| Affiliation: | 1.Soybean Research Institute of Nanjing Agricultural University/National Center for Soybean Improvemet/State Key Laboratory of Crop Genetics and Germplasm Enhancement， Nanjing 210095，China；2 State Key Laboratory of Plant Gemomics，Institute of Genetics and developmental Biology,Chinese Academy of Sciences,Being 100101,China |
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| Abstract: | A high-density genetic linkage map with informative markers is essential for plant genome analysis, including gene mapping, identification of quantitative trait locus (QTL), map-based cloning, and physical map construction. Though four genetic linkage maps for the recombinant inbred line population NJRIKY derived from (Kefeng 1×Nannong 1138-2) have been established already, there are still problems of precision and accuracy in mapping genes and QTLs due to insufficiency of genetic information and number of markers. A total of 401 polymorphic SSR markers were screened out from 967 ones for density-enhancement of the previous maps. Along with other marker data, a new genetic linkage map was constructed by using Mapmaker/Exp 3.0b, with 553 markers, including 316 SSR, 197 RFLP, 39 EST and one morphologic markers, spanning 25 linkage groups, covering total length 2 071.6 cM of the soybean genome, with an average marker interval distance of 3.70 cM. In comparison with the old map, the number of gaps larger than 20 cM decreased from forty-two to two on the enhanced map. Using this map to relocate the seven SMV resistant genes, Rsc-3, Rsc-7, Rsc-9, Rsc-13, Rsa, Rn1 and Rn3 were mapped on LG D1b again with distances to the flanking markers all less than 6 cM, among them, Rsc-9, Rn1 and Rsa less than 1 cM and Rsc-13 co-segregating with EST-SSR marker GMKF168a. After re-mapping the QTLs for eight agronomic traits, 42 QTLs were detected on 12 linkage groups, with 20 of them accounted for more than 10% of the total variation, respectively, and their marker intervals obviously shortened. |
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| Keywords: | Soybean Genetic linkage map SSR markers QTL |
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