| 一株鸡马立克氏病病毒的分离鉴定及主要致病基因分析 |
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| 引用本文： | 李思菲,孙鹏,陈俊霞,秦魁,苏帅,赵鹏,崔治中. 一株鸡马立克氏病病毒的分离鉴定及主要致病基因分析[J]. 中国畜牧兽医, 2016, 43(5): 1343-1348. DOI: 10.16431/j.cnki.1671-7236.2016.05.033 |
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| 作者姓名： | 李思菲 孙鹏 陈俊霞 秦魁 苏帅 赵鹏 崔治中 |
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| 作者单位： | 1. 山东农业大学动物医学院, 泰安 271018;2. 烟台市福山区动物卫生监督所, 烟台 265500 |
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| 基金项目： | 国家自然科学基金(31072149) |
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| 摘 要： | 山东省某地区鸡马立克氏病疫苗免疫鸡群暴发马立克氏病(MD),为分离得到致病毒株,检测其致病性,采用琼脂扩散试验、细胞培养和间接免疫荧光试验(IFA)等方法从发病鸡的血液及羽髓中分离到一株适应鸡胚成纤维细胞(CEF)生长的马立克氏病病毒。采用PCR方法扩增分离毒株的meq、pp38、132bp重复序列等病毒致病相关基因,所得序列用DNAStar软件与GenBank上登录的参考毒株进行比对分析。结果显示,该分离株SDAU-1的pp38基因与标准强毒序列同源性为100%,132bp重复序列的拷贝数及meq基因的变异均符合MDV强毒株的序列特征。
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| 关 键 词： | 马立克氏病病毒 分离 鉴定 序列比对 meq基因 |
| 收稿时间： | 2015-10-29 |
Isolation and Identification of a Field Strain of Marek's Disease Virus and Analysis of Major Pathogenic Genes |
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| LI Si-fei,SUN Peng,CHEN Jun-xia,QIN Kui,SU Shuai,ZHAO Peng,CUI Zhi-zhong. Isolation and Identification of a Field Strain of Marek's Disease Virus and Analysis of Major Pathogenic Genes[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(5): 1343-1348. DOI: 10.16431/j.cnki.1671-7236.2016.05.033 |
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| Authors: | LI Si-fei SUN Peng CHEN Jun-xia QIN Kui SU Shuai ZHAO Peng CUI Zhi-zhong |
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| Affiliation: | 1. Animal Medical College of Shandong Agricultural University, Tai'an 271018, China;2. Institute of Animal Health Supervision of Fushan District, Yantai City, Yantai 265500, China |
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| Abstract: | In a certain area of Shandong province, Marek's disease (MD) occurred in diseased chickens that had been vaccinated by turkey herpesvirus.In order to isolate the virus strain and detect the virus pathogenicity, agar diffusion test, cell culture and indirect immunofluorescence assay (IFA) were used to isolate the Marek's virus from chicken's blood and feather marrow.The isolated strain was adapted to grow in chick embryo fibroblasts (CEF).Genes involved in pathogenesis of MDV, such as meq, pp38 and 132 bp repeat sequence were amplified by PCR.The obtained sequences were compared with that of standard strains published in GenBank by DNAStar software.The results showed that pp38 gene of the SDAU-1 shared homology from 100% with standard virulent sequence.Analysis of 132 bp repeat sequence and meq gene sequences of the viral genome showed that the isolated virus belongs to the highly virulent MDV strains. |
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| Keywords: | Marek's disease virus isolation identification sequence comparison meq gene |
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