| 微波加热快速提取芽孢杆菌与乳酸菌DNA |
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| 引用本文： | 周晏阳,邱莞思,唐俊妮,汤承. 微波加热快速提取芽孢杆菌与乳酸菌DNA[J]. 中国畜牧兽医, 2016, 43(10): 2792-2796. DOI: 10.16431/j.cnki.1671-7236.2016.10.042 |
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| 作者姓名： | 周晏阳 邱莞思 唐俊妮 汤承 |
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| 作者单位： | 西南民族大学生命科学与技术学院, 成都 610041 |
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| 基金项目： | “十二五”科技支撑计划（2012BAD13B06） |
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| 摘 要： | 试验旨在研究快速、简单、高效提取芽孢杆菌与乳酸菌DNA的方法。在微波炉额定功率800 W下，对芽孢杆菌和乳酸菌进行微波处理40~150 s，离心获取上清，收获细菌DNA，将样本DNA PCR扩增后进行琼脂糖凝胶电泳验证，测定其纯度和产量后测序。结果显示，在微波炉功率800 W条件下加热40~150 s均可有效提取芽孢杆菌与乳酸菌DNA。所得DNA质量较好（D260 nm/D280 nm在1.8~2.1之间）。DNA浓度符合PCR检测要求，目的条带清晰，所得测序结果满足常规分子生物学研究。该微波提取方法具有简单、快速、高效的特点，且具有广泛的实用性，为乳酸菌与芽孢杆菌快速分子检测提供了简便手段。
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| 关 键 词： | 微波炉加热 提取DNA 芽孢杆菌 乳酸菌 |
| 收稿时间： | 2016-03-29 |
Rapid Extraction of Bacillus and Lactobacillus DNA by Microwave Heating |
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| ZHOU Yan-yang,QIU Wan-si,TANG Jun-ni,TANG Cheng. Rapid Extraction of Bacillus and Lactobacillus DNA by Microwave Heating[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(10): 2792-2796. DOI: 10.16431/j.cnki.1671-7236.2016.10.042 |
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| Authors: | ZHOU Yan-yang QIU Wan-si TANG Jun-ni TANG Cheng |
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| Affiliation: | College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China |
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| Abstract: | The assay was aimed to investigate a fast,simple and efficient method with microwave heating for extracting DNA of Bacillus and Lactobacillus.Treated in a microwave oven at rated power (800 W) for a certain time (40 to 150 s),then centrifuged for supernatant.The supernatant was transferred to a sterile tube for PCR testing and agarose gel electrophoresis.Sequencing was carried out after determining the purity and yield.The results showed that heating for 40 to 150 s at 800 W microwave power conditions could effectively extract DNA of Bacillus and Lactobacillus.The obtained DNA quality was good (D260 nm/D280 nm was 1.8 to 2.1).The DNA concentration in line with the PCR testing requirements;Sequencing results was satisfied with the routine molecular biological research.The microwave extraction method had simple,fast and efficient features,and a wide range of practical,which provided a simple means for rapid molecular assay. |
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| Keywords: | microwave heating DNA extraction Bacillus Lactobacillus |
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