| GnIH与INH表位多肽疫苗主动免疫对甘肃高山细毛羊生殖激素的影响 |
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| 引用本文： | 张玲玲,杨博辉,岳耀敬,郭婷婷,刘建斌,袁超,牛春娥,冯瑞林,郭健,孙晓萍,刘善博. GnIH与INH表位多肽疫苗主动免疫对甘肃高山细毛羊生殖激素的影响[J]. 中国畜牧兽医, 2016, 43(4): 1039-1044. DOI: 10.16431/j.cnki.1671-7236.2016.04.028 |
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| 作者姓名： | 张玲玲 杨博辉 岳耀敬 郭婷婷 刘建斌 袁超 牛春娥 冯瑞林 郭健 孙晓萍 刘善博 |
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| 作者单位： | 中国农业科学院兰州畜牧与兽药研究所, 兰州 730050 |
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| 基金项目： | 甘肃省青年自然基金;非繁殖季节GnIH基因免疫对藏羊卵泡发育的调节机制;国家绒毛用羊产业技术体系;分子育种岗位(NYCY+X-40-3);中国农业科学院羊育种工程研究中心 |
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| 摘 要： | 本试验通过合成促性腺激素类的抑制激素——抑制素(inhibit hormone,INH)与促性腺激素抑制激素(gonadotropin inhibiting hormone,GnIH)的INH表位多肽疫苗和GnIH表位多肽疫苗,主动免疫甘肃高山细毛羊并对促卵泡素(follicle stimulating hormone,FSH)和促黄体素(luteinizing hormone,LH)激素水平进行比较分析。试验选用15只处在非发情季节的3.5岁的甘肃高山细毛羊,注射INH表位多肽疫苗与GnIH表位多肽疫苗分别为表位多肽疫苗A组和表位多肽疫苗B组,对照组注射等量生理盐水。采血后进行免疫,每隔7 d免疫和采血,同时在发情后每隔15 min采集一次血液,分离血清,用ELISA试剂盒检测抗体水平及FSH和LH激素变化。结果表明,表位多肽疫苗A组和表位多肽疫苗B组在初次免疫后都产生了相应的抗体,且表位多肽疫苗B组较表位多肽疫苗A组产生的抗体浓度高,75 、90、105 min时,表位多肽疫苗B组相对表位多肽疫苗A组显著促进了FSH的分泌水平(P<0.05)。在45~105 min时,表位多肽疫苗B组相对表位多肽疫苗A组显著促进了LH的分泌水平(P<0.05),且在90到105 min时表位多肽疫苗A组和表位多肽疫苗B组血清中FSH和LH的水平均显著高于对照组(P<0.05)。本试验结果表明,INH表位多肽疫苗和GnIH表位多肽疫苗主动免疫促进了甘肃高山细毛羊的FSH、LH的分泌,GnIH表位多肽疫苗在甘肃高山细毛羊上具有更好的免疫作用,本研究为该表位多肽疫苗在绵羊上的运用奠定了基础。
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| 关 键 词： | 促性腺激素抑制激素 抑制素 主动免疫 甘肃高山细毛羊 生殖激素 |
| 收稿时间： | 2015-07-31 |
Effects of Active Immunization with GnIH and INH Epitope Peptide Vaccine on Reproductive Hormones of Gansu Alpine Fine-wool Sheep |
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| ZHANG Ling-ling,YANG Bo-hui,YUE Yao-jing,GUO Ting-ting,LIU Jian-bin,YUAN Chao,NIU Chun-e,FENG Rui-lin,GUO Jian,SUN Xiao-ping,LIU Shan-bo. Effects of Active Immunization with GnIH and INH Epitope Peptide Vaccine on Reproductive Hormones of Gansu Alpine Fine-wool Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2016, 43(4): 1039-1044. DOI: 10.16431/j.cnki.1671-7236.2016.04.028 |
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| Authors: | ZHANG Ling-ling YANG Bo-hui YUE Yao-jing GUO Ting-ting LIU Jian-bin YUAN Chao NIU Chun-e FENG Rui-lin GUO Jian SUN Xiao-ping LIU Shan-bo |
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| Affiliation: | Lanzhou Institute of Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China |
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| Abstract: | This study aimed to detect the reproductive hormones levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in Gansu Alpine Fine-wool sheep by active immunization of two synthesized epitope peptide vaccines A and B which were combined inhibit hormone (INH) and gonadotropin inhibit hormone (GnIH).15 Gansu Alpine Fine-wool sheep were selected which were 3.5 year old and lived in anoestrus season, 3 groups each had 5 sheep, GnIH epitope peptide vaccine and INH epitope peptide vaccine were named epitope peptide vaccine A and B groups, while blank group was injected with saline.The blood was collected before immunization and immuned, and bloods were collected once every 7 days, meanwhile collected blood every 15 min after estrus.The serum was used to detect antibody, FSH and LH levels by ELISA Kits.The results showed that the epitope peptide vaccine A and B groups produced the corresponding antibodies after the initial immunization, and epitope peptide vaccine B group produced more antibodies than epitope peptide vaccine A group.Epitope peptide vaccine B group promoted the FSH secretion levels was significantly higher than epitope peptide vaccine A group in 75, 90 and 105 min (P<0.05);In 45 to 105 minutes, the LH secretion levels of epitope peptide vaccine B group was significantly higher than epitope peptide vaccine A group (P<0.05), while the LH and FSH levels in epitope peptide vaccine A and B groups were significantly higher than control group (P<0.05).This study indicated that the epitope peptide vaccine A and B groups could promote the FSH and LH secretion levels through active immunity in Gansu Alpine Fine-wool sheep and had a good immune function.This study laid a foundation for the application of epitope peptide vaccine on sheep. |
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| Keywords: | gonadotropin inhibit hormone (GnIH) inhibit hormone (INH) active immune Gansu Alpine Fine-wool sheep reproductive hormones |
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