| 巴什拜羊微卫星标记多态性及其与生长指标关联性分析 |
| |
| 引用本文： | 决肯·阿尼瓦什,韩业东,李齐发,帕娜尔·依都拉,谢庄. 巴什拜羊微卫星标记多态性及其与生长指标关联性分析[J]. 中国农业科学, 2010, 43(16): 3425-3432. DOI: 10.3864/j.issn.0578-1752.2010.16.019 |
| |
| 作者姓名： | 决肯·阿尼瓦什 韩业东 李齐发 帕娜尔·依都拉 谢庄 |
| |
| 作者单位： | (南京农业大学动物科技学院) |
| |
| 基金项目： | 国家自然科学基金资助项目，新疆自治区科技厅攻关项目，新疆自治区科技厅重大专项 |
| |
| 摘 要： | 【目的】以巴什拜羊为研究材料,通过分析微卫星DNA座位的基因型,并与生长性状进行相关分析,以寻找影响巴什拜羊生长性状的微卫星标记,为分子标记辅助选择提供依据。【方法】从GenBank数据库查询绵羊、山羊的微卫星座位,根据微卫星选择标准选择符合要求的10个微卫星标记,对巴什拜羊189个个体基因组DNA进行检测,采用最小二乘方法分析微卫星座位与生长指标的关联效应。【结果】10个微卫星标记共检测到110个等位基因,平均每个座位等位基因数为11个,平均多态信息含量为0.7914,平均杂合度为0.8149;10个微卫星座位中有8个微卫星座位与巴什拜羊体重、体尺等5项生长指标有显著和极显著的关联性,特别是基因座BM415与体重、体尺指标均呈极显著相关(P0.01),多重比较结果显示8个微卫星座位都存在优势基因型。【结论】发现了巴什拜羊群体生长指标具有显著效应,多态性较高的微卫星基因座,为开展巴什拜羊生长指标的分子标记辅助育种提供了有价值的遗传标记。
|
| 关 键 词： | 巴什拜羊 微卫星标记 遗传多样性 生长指标 关联性 |
| 收稿时间： | 2010-04-19; |
Polymorphic Analysis on Microsatellite Marker and Its Growth Index Association of Bashbay Sheep |
| |
| ANIWASHI J K,HAN Ye-dong,LI Qi-fa,YIDULA P N E,XIE Zhuang. Polymorphic Analysis on Microsatellite Marker and Its Growth Index Association of Bashbay Sheep[J]. Scientia Agricultura Sinica, 2010, 43(16): 3425-3432. DOI: 10.3864/j.issn.0578-1752.2010.16.019 |
| |
| Authors: | ANIWASHI J K HAN Ye-dong LI Qi-fa YIDULA P N E XIE Zhuang |
| |
| Affiliation: | (College of Animal Science and Technology of Nanjing Agricultural University)
|
| |
| Abstract: | 【Objective】 Taking Bashbay sheep as research material, this paper aimed at seeking the microsatellite marker which influenced the growth property of Bashbay sheep through analysis on the genotype of microsatellite DNA locus and growth property, and thus providing a basis for the molecular marker assisted selection. 【Method】 Inquiried about the microsatellite locus of sheep and goat from GenBank database, 10 microsatellite markers that meet the requirements of the microsatellite standard were selected, 189 individual genome DNA of sheep were detected, and the correlated effects of microsatellite locus and growth indexes were analyzed by adopting least-squares method. 【Result】 There were 110 allelic genes were detected by 10 microsatellite markers, with an average of 11 allelic genes for each, the average polymorphic information content was 0.7914, the average heterozygosity was 0.8149. There were 8 of 10 microsatellite locus had significant and very significant relation with 5 growth indexes including weight and size of Bashbay sheep, especially the significant correlation of gene locus BM415 and weight and size (P＜0.01). Multiple comparative results showed that 8 microsatellite locus had genotype advantage.【Conclusion】 Significant effect was found in the growh indexes of Bashbay sheep group, and the higher microsatellite locus offered valuable genetic markers for developing the molecular marker assisted breeding of growth indexes of Bashbay sheep. |
| |
| Keywords: | Bashbay sheep microsatellite markers genetic diversity growth indexes association |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
|
点击此处可从《中国农业科学》下载全文 |
|
