| 茶树EST-SNP分布特征及标记开发 |
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| 引用本文： | 王丽鸳,张成才,成浩,韦康. 茶树EST-SNP分布特征及标记开发[J]. 茶叶科学, 2012, 32(4): 369-376. DOI: 10.13305/j.cnki.jts.2012.04.014 |
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| 作者姓名： | 王丽鸳 张成才 成浩 韦康 |
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| 作者单位： | 中国农业科学院茶叶研究所,国家茶树改良中心,浙江 杭州 310008 |
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| 基金项目： | 现代农业产业技术体系建设专项资金，浙江省自然科学基金，浙江省茶产业技术创新战略联盟专项资金 |
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| 摘 要： | 对NCBI网上公开的12757条茶树ESTs序列进行聚类,成功构建了茶树的独立基因（Unigene）数据库,发现茶树的EST序列冗余率约为68.2%。初步建立了茶树EST-SNP标记开发体系,明确了茶树EST中SNP的分布规律,茶树编码区的SNP发生频率约为0.58%,平均200bp就有1个SNP位点,并进一步推算出茶树基因组DNA序列的杂合率约为0.38%,平均300个碱基就可能出现1个杂合位点。从237个多基因聚类簇中发现了818个SNP候选位点,设计了25对SNP引物进行DNA重测序验证,发现EST-SNP候选位点的多态检出率为75%。
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| 关 键 词： | 茶树 表达序列标签 单核苷酸序列多态性 |
| 收稿时间： | 2011-11-02 |
Characterization of EST-derived SNPs and Development of SNP-markers in Tea (Camellia sinensis) |
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| WANG Li-yuan , ZHANG Cheng-cai , CHENG Hao , WEI Kang. Characterization of EST-derived SNPs and Development of SNP-markers in Tea (Camellia sinensis)[J]. Journal of Tea Science, 2012, 32(4): 369-376. DOI: 10.13305/j.cnki.jts.2012.04.014 |
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| Authors: | WANG Li-yuan ZHANG Cheng-cai CHENG Hao WEI Kang |
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| Affiliation: | Tea Research Institute, Chinese Academy of Agricultural Sciences; National Center for Tea Improvement, Hangzhou 310008, China |
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| Abstract: | By clustering the 12 757 ESTs of tea downloaded from NCBI,a unigene database of tea was successfully built.It was found that the redundancy rate for ESTs from tea was approximately 68.2%.An EST-SNP exploiting system for tea was established preliminarily.The SNP distribution was identified.The occurrence frequency for coding region SNP in tea was appropriately 0.58%.It meant that there was average one SNP in every 200 bp of tea ESTs.Furthermore,the hybrid rate for tea genome was deduced to be 0.38%,averagely one hybrid DNA site per 300 bp.Totally 818 candidated SNP were exploited from 237 multigene clusters.Then 25 pairs of SNP primers were designed and 75% of these sites were validated to be polymorphism by DNA sequencing. |
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| Keywords: | tea plant Camellia sinensis EST SNP |
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