| 黑土区大豆基因型的根际细菌群落结构时空动态变化 |
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| 引用本文： | JIN Jian,WANG Guang-Hu,LIU Xiao-Bing,LIU J&#;-Dong,CHEN Xue-Li,S. J. HERBERT. 黑土区大豆基因型的根际细菌群落结构时空动态变化[J]. 土壤圈, 2009, 19(6): 808-816. DOI: 10.1016/S1002-0160(09)60176-4 |
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| 作者姓名： | JIN Jian,WANG Guang-Hu,LIU Xiao-Bing,LIU J&# -Dong,CHEN Xue-Li,S. J. HERBERT |
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| 作者单位： | JIN Jian,WANG Guang-Hua,LIU Xiao-Bing,LIU Jü-Dong,CHEN Xue-Li(Key Lab of Black Soil Ecology,Northeast Institute of Geography and Agricultural Ecology,Chinese Academy of Sciences,Harbin 150081 China);S.J.HERBERT(Department of Plant,Soil,and Insect Sciences,University of Massachusetts,Amherst MA 01003 USA) |
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| 基金项目： | *1Project supported by the National Natural Science Foundation of China (Nos. 40671099 and 40701084) and the Director Program of the Key Laboratory of Soybean Biology of Ministry of Education, China (No. SB05B02). |
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| 摘 要： | The dynamics of rhizosphere microbial communities is important for plant health and productivity, and can be influenced by soil type, plant species or genotype, and plant growth stage. A pot experiment was carried out to examine the dynamics of microbial communities in the rhizosphere of two soybean genotypes grown in a black soil in Northeast China with a long history of soybean cultivation. The two soybean genotypes, Beifeng 11 and Hai 9731, differing in productivity were grown in a mixture of black soil and siliceous sand. The bacterial communities were compared at three zone locations including rhizoplane, rhizosphere, and bulk soil at the third node (V3), early flowering (R1), and early pod (R3) stages using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA. The results of principal component analyses (PCA) showed that the bacterial community structure changed with growth stage. Spatially, the bacterial communities in the rhizoplane and rhizosphere were significantly different from those in the bulk soil. Nevertheless, the bacterial communities in the rhizoplane were distinct from those in the rhizosphere at the V3 stage, while no obvious differences were found at the R1 and R3 stages. For the two genotypes, the bacterial community structure was similar at the V3 stage, but differed at the R1 and R3 stages. In other words, some bacterial populations became dominant and some others recessive at the two later stages, which contributed to the variation of the bacterial community between the two genotypes. These results suggest that soybean plants can modify the rhizosphere bacterial communities in the black soil, and there existed genotype-specific bacterial populations in the rhizosphere, which may be related to soybean productivity.
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| 关 键 词： | PCR-DGGE plant growth stage 16S rDNA soybean productivity |
| 收稿时间： | 2008-10-15 |
Temporal and spatial dynamics of bacterial community in the rhizosphere of soybean genotypes grown in a black soil |
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| JIN Jian,WANG Guang-Hu,LIU Xiao-Bing,LIU J,#;-Dong,CHEN Xue-Li and S. J. HERBERT. Temporal and spatial dynamics of bacterial community in the rhizosphere of soybean genotypes grown in a black soil[J]. Pedosphere, 2009, 19(6): 808-816. DOI: 10.1016/S1002-0160(09)60176-4 |
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| Authors: | JIN Jian,WANG Guang-Hu,LIU Xiao-Bing,LIU J&# -Dong,CHEN Xue-Li S. J. HERBERT |
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| Affiliation: | Key Lab of Black Soil Ecology, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150081 (China);Key Lab of Black Soil Ecology, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150081 (China);Key Lab of Black Soil Ecology, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150081 (China);Key Lab of Black Soil Ecology, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150081 (China);Key Lab of Black Soil Ecology, Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Harbin 150081 (China);Department of Plant, Soil, and Insect Sciences, University of Massachusetts, Amherst MA 01003 (USA) |
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| Abstract: | The dynamics of rhizosphere microbial communities is important for plant health and productivity, and can be influenced by soil type, plant species or genotype, and plant growth stage. A pot experiment was carried out to examine the dynamics of microbial communities in the rhizosphere of two soybean genotypes grown in a black soil in Northeast China with a long history of soybean cultivation. The two soybean genotypes, Beifeng 11 and Hai 9731, differing in productivity were grown in a mixture of black soil and siliceous sand. The bacterial communities were compared at three zone locations including rhizoplane, rhizosphere, and bulk soil at the third node (V3), early flowering (R1), and early pod (R3) stages using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rDNA. The results of principal component analyses (PCA) showed that the bacterial community structure changed with growth stage. Spatially, the bacterial communities in the rhizoplane and rhizosphere were significantly different from those in the bulk soil. Nevertheless, the bacterial communities in the rhizoplane were distinct from those in the rhizosphere at the V3 stage, while no obvious differences were found at the R1 and R3 stages. For the two genotypes, the bacterial community structure was similar at the V3 stage, but differed at the R1 and R3 stages. In other words, some bacterial populations became dominant and some others recessive at the two later stages, which contributed to the variation of the bacterial community between the two genotypes. These results suggest that soybean plants can modify the rhizosphere bacterial communities in the black soil, and there existed genotype-specific bacterial populations in the rhizosphere, which may be related to soybean productivity. |
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| Keywords: | PCR-DGGE plant growth stage 16S rDNA soybean productivity |
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