| 枯草芽孢杆菌Czk1挥发物混合活性组分对橡胶灵芝菌的抑菌机理 |
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| 引用本文： | 梁艳琼,李锐,吴伟怀,习金根,谭施北,黄兴,陆英,贺春萍,易克贤. 枯草芽孢杆菌Czk1挥发物混合活性组分对橡胶灵芝菌的抑菌机理[J]. 中国农业科技导报, 2022, 24(2): 152-159. DOI: 10.13304/j.nykjdb.2020.0991 |
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| 作者姓名： | 梁艳琼 李锐 吴伟怀 习金根 谭施北 黄兴 陆英 贺春萍 易克贤 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所，农业农村部热带作物有害生物综合治理重点实验室，海南省热带农业有害生物检测监控重点实验室，海口 571101 |
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| 基金项目： | 海南省自然科学基金面上项目(320MS083);海南省科协青年科技英才学术创新计划项目(QCXM201714);国家天然橡胶产业技术体系建设项目(CARS-33-BC1);海南省院士创新平台科研专项 |
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| 摘 要： | 红根病是我国分布广、发病率高、危害较为严重的橡胶树根部病害.为明确枯草芽孢杆菌Czk1菌株产生的挥发性物质对橡胶红根病原菌橡胶灵芝菌(Ganoderma pseudoferreum)的抑菌作用,以挥发性物质的主要成分苯甲醛、3,5-二.甲氧基苯乙酮、2,6-二叔丁基对甲酚为研究对象,分析其对橡胶灵芝菌的糖、蛋白、DNA...
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| 关 键 词： | 枯草芽孢杆菌 橡胶灵芝菌 挥发物活性组分 抑菌机理 |
| 收稿时间： | 2020-11-26 |
Inhibitory Mechanism of Volatilized Active Components from Bacillus subtilis Czk1 on Ganoderma pseudoferreum |
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| Yanqiong LIANG,Rui LI,Weihuai WU,Jingen XI,Shibei TAN,Xing HUANG,Ying LU,Chunping HE,Kexian YI. Inhibitory Mechanism of Volatilized Active Components from Bacillus subtilis Czk1 on Ganoderma pseudoferreum[J]. Journal of Agricultural Science and Technology, 2022, 24(2): 152-159. DOI: 10.13304/j.nykjdb.2020.0991 |
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| Authors: | Yanqiong LIANG Rui LI Weihuai WU Jingen XI Shibei TAN Xing HUANG Ying LU Chunping HE Kexian YI |
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| Affiliation: | Key Laboratory of Integrated Pest Management on Tropical Crops，Ministry of Agriculture and Rural Affairs； Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests； Environment and Plant Protection Institute，Chinese Academy of Tropical Agricultural Sciences，Haikou 571101，China |
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| Abstract: | Red root disease is the root disease of rubber trees with wide distribution， high incidence， and more harmful in China. In order to determine the inhibitory effect of volatilized active components produced by Bacillus subtilis Czk1 strain on Ganoderma pseudoferreum， the dominant of active components， benzaldehyde， 3，5-dimethoxyacetophenone and butylated hydroxytoluene were used as research subjects to analyze their effects on sugar， protein， DNA， activity of key metabolic enzymes and cell content leakage of G. pseudoferreum. The results showed after G. pseudoferreum was treated with the active components of volatiled organic compounds， the sugar content of G. pseudoferreum decreased significantly， and the volatilized active components with high concentration affected the protein synthesis of G. pseudoferreum； the molecular weight of DNA did not change significantly， indicating the primary structure of DNA was not destoried； the activities of key metabolic enzymes （malate dehydrogenase and chitinase） decreased， and the leakage of intracellular lysates was obvious. The active components of volatile organic compounds achieved bacteriostasis by destroying the cell membrane structure and disrupting the metabolic activity of G. pseudoferreum. |
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| Keywords: | Bacillus subtilis Czk1 Ganoderma pseudoferreum volatilized active component inhibitory mechanism |
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