| 基于提高CRISPR/Cas基因编辑效率的研究进展 |
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| 引用本文： | 张玉苗,李蓉,鲁瑶,林玉玲,赖钟雄,徐涵. 基于提高CRISPR/Cas基因编辑效率的研究进展[J]. 热带作物学报, 2019, 40(10): 2006-2015. DOI: 10.3969/j.issn.1000-2561.2019.10.013 |
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| 作者姓名： | 张玉苗 李蓉 鲁瑶 林玉玲 赖钟雄 徐涵 |
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| 作者单位： | 1. 滨州学院生物与环境工程学院/山东省黄河三角洲生态环境重点实验室,山东滨州 2566002. 福建农林大学园艺植物生物工程研究所,福州 3500023. 法国图卢兹综合科学研究所,图卢兹 31300 |
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| 基金项目： | 山东省自然科学基金项目(ZR2017PC010);国家自然科学基金项目(31572088);福建省高原学科建设经费(102/71201801101) |
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| 摘 要： | CRISPR基因编辑技术发展迅速,为功能基因的研究及遗传改良提供了技术支持。提高基因组编辑效率成为使用基因编辑技术的基本点,目前已取得了重要进展。本文结合CRISPR/Cas系统的作用原理、晶体结构,着重介绍目前提高编辑效率的最新研究进展,对CRISPR/Cas基因编辑效率的提高进行分析并提出改进策略。
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| 关 键 词： | CRISPR/Cas 基因组编辑 基因编辑效率 |
| 收稿时间： | 2019-07-30 |
Research Progress on Improving CRISPR/Cas Genome Editing Efficiency |
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| ZHANG Yumiao,LI Rong,LU Yao,LIN Yuling,LAI Zhongxiong,XU Han. Research Progress on Improving CRISPR/Cas Genome Editing Efficiency[J]. Chinese Journal of Tropical Crops, 2019, 40(10): 2006-2015. DOI: 10.3969/j.issn.1000-2561.2019.10.013 |
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| Authors: | ZHANG Yumiao LI Rong LU Yao LIN Yuling LAI Zhongxiong XU Han |
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| Affiliation: | 1. College of Biological and Environmental Engineering / Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou University, Binzhou, Shandong 256600, China2. Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China3. Institut de la Recherche Interdisciplinaire de Toulouse, IRIT-ARI, Toulouse 31300, France |
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| Abstract: | CRISPR genome editing technology has developed rapidly and provided technical support for the functional genes research and genetic improvement. High efficiency of genome editing is critical for the application of the technology, and important progresses have been made recently. The present review summarized historic events in the improvement of the technology especially of the editing efficiency in relation to the functional mechanism and structure of the CRISPR/Cas system, and proposed several technological hints to the improvement of the editing efficiency. |
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| Keywords: | CRISPR/Cas genome editing genome editing efficiency |
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