| 桐花树根多糖的提取、理化性质及其抗酪氨酸酶活性研究 |
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| 引用本文： | 蔡建秀,顾雅青,黄晓冬,葛清秀,黄建新,李元跃,林彩春. 桐花树根多糖的提取、理化性质及其抗酪氨酸酶活性研究[J]. 中国农学通报, 2015, 31(31): 12-18. DOI: 10.11924/j.issn.1000-6850.casb15070067 |
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| 作者姓名： | 蔡建秀 顾雅青 黄晓冬 葛清秀 黄建新 李元跃 林彩春 |
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| 作者单位： | 泉州师范学院化生学院,泉州师范学院化生学院,泉州师范学院近海资源生物技术福建省高校重点实验室,泉州师范学院化生学院,福建医科大学附属第二医院;福建医科大学附属第二医院,福建省海洋渔业资源与生态环境重点实验室,泉州师范学院近海资源生物技术福建省高校重点实验室 |
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| 基金项目： | ：2015 年福建省（国家级）大学生创新创业训练计划项目“桐花树叶多酚提取纯化及抗衰老功能化妆品研制和成分分析”(201410399013)；福建省自然科学基金项目“红树植物桐花树叶片抗炎镇痛有效部位的开发”(2015J01146)；福建省海洋渔业资源与生态环境重点实验室开放基金资助项目“红树植物桐花树叶片抗炎镇痛活性部位及其化学成分研究”(fmfre2014011)；泉州市科技计划B类项目“桐花树提取物防治骨性关节炎的药效学与机制研究”(Z20140156)；福建省生物学重点学科专项经费，泉州师范学院“海洋功能食品工程中心”资助。 |
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| 摘 要： | 旨在探究桐花树根多糖的理化性质和抗酪氨酸酶活性。以水提法、酸提法、碱提法连续提取桐花树根多糖，经脱脂脱色、脱蛋白、透析等方法得到初步纯化的多糖，紫外检测与显色反应分析其理化性质；探究多糖对酪氨酸酶活性的影响。结果表明：桐花树根水提物的多糖（中性多糖）含量为34.02%，酸提物多糖（酸性多糖）含量为27.18%，碱提物的多糖（碱性多糖）含量为51.21%。在浓度0.2~1.0 mg/mL时，桐花树根中性多糖、碱性多糖对酪氨酸酶活性具有双向调节作用，当浓度小于0.4 mg/mL时，对酪氨酸酶活性具有抑制作用，当浓度大于0.6 mg/mL时，表现为激活作用；但酸性多糖对酪氨酸酶活性仅表现为激活作用。
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| 关 键 词： | 预测模型 预测模型 土壤容重 土壤传递函数 BP神经网络 双参数圆锥指数仪 |
| 收稿时间： | 2015-07-11 |
| 修稿时间： | 2015-10-05 |
Study on Extraction, Physicochemical Properties and Anti-tyrosinase Activity of Polysaccharides |
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| Abstract: | To study the physicochemical properties and anti-tyrosinase activity of polysaccharide in Aegiceras corniculatum root, the crude polysaccharides were extracted from Aegiceras corniculatum roots by the sequential extraction methods of water extraction, acid extraction and alkaline extraction. The crude polysaccharides were depurated by degreasing, decoloration, deproteinization and dialysis, its physicochemical properties were examined by UV analysis and various chromogenic reactions, the effects on tyrosinase activity were also researched. The results were as follows: the polysaccharide (neutral polysaccharide) content of aqueous extract from Aegiceras corniculatum roots was 34.02%, acidic polysaccharose content of acid extract was 27.18% , and alkaline polysaccharide content of alkali extract was 51.21% . At the polysaccharose concentration of 0.2-1.0 mg/mL, it was found that the neutral polysaccharide and acidic polysaccharose had dual-directional regulation effects on tyrosinase activity, which showed the inhibition effect under 0.4 mg/mL and activation effect above 0.6 mg/mL, but acidic polysaccharose only showed activation effect. |
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| Keywords: | Aegiceras corniculatum root polysaccharide extraction physicochemical properties tyrosinase |
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