| 鸡粪好氧堆肥过程中氨氧化古菌群落结构的动态变化 |
| |
| 引用本文： | 解开治,徐培智,张发宝,唐拴虎,顾文杰,黄旭,蒋瑞萍,卢钰升. 鸡粪好氧堆肥过程中氨氧化古菌群落结构的动态变化[J]. 植物营养与肥料学报, 2012, 18(6): 1487-1494. DOI: 10.11674/zwyf.2012.12159 |
| |
| 作者姓名： | 解开治 徐培智 张发宝 唐拴虎 顾文杰 黄旭 蒋瑞萍 卢钰升 |
| |
| 作者单位： | 1.广东省农业科学院土壤肥料研究所,广州 510640; |
| |
| 基金项目： | 中央农业科技成果转化资金项目，广东省重点科研基地建设项目，广东省现代农业产业技术体系专项资助 |
| |
| 摘 要： | 应用聚合酶链式反应-变性梯度凝胶电泳（PCR DGGE）技术，研究了好氧堆肥过程氨氧化古菌（ammonia oxidizing archaea, AOA）的群落结构和多样性变化。结果表明，不同堆肥时期鸡粪好氧堆肥AOA菌群的群落结构发生了明显的变化。与AOA HH 2(GU2258721)亲缘关系较近的b条带(相似性96%)和未培养泉古菌属［uncultured crenarchaeote NM 152(HQ8752251)］的m条带(相似性99%)是堆肥过程一直存在的AOA菌属。条带c、 b、 f、 i和条带m、 k、 l、 n代表的种属分属两个大类。AOA群落的Shannon Weiner指数（H）、 均匀度指数（EH）均表现为第30 d＞第5 d＞第25 d第45 d＞第3 d第12 d＞第1 d第15 d。对AOA种群数据与不同时期堆体温度、 pH、 全氮、 铵态氮、 亚硝态氮和硝态氮等环境因子的冗余分析表明，堆体温度、 全氮、 亚硝态氮和硝态氮对氨氧化古菌群落演替有着显著的影响（P0.05），AOA的群落结构在堆肥第1 d、 第5 d、 第25 d、 第30 d、 第45 d变化较大。说明这些参数能有效控制鸡粪好氧堆肥过程中AOA的群落结构。
|
| 关 键 词： | 好氧堆肥 氨氧化古菌 PCR-DGGE 群落结构 |
| 收稿时间： | 2012-04-06 |
Dynamic changes of ammonia-oxidizing archaea community structure during aerobic composting of chicken manure |
| |
| JIE Kai-zhi XU Pei-zhi ZHANG Fa-bao TANG Shuan-hu GU Wen-jie HUANG Xu JIANG Rui-ping LU Yu-shen. Dynamic changes of ammonia-oxidizing archaea community structure during aerobic composting of chicken manure[J]. Plant Nutrition and Fertilizer Science, 2012, 18(6): 1487-1494. DOI: 10.11674/zwyf.2012.12159 |
| |
| Authors: | JIE Kai-zhi XU Pei-zhi ZHANG Fa-bao TANG Shuan-hu GU Wen-jie HUANG Xu JIANG Rui-ping LU Yu-shen |
| |
| Affiliation: | 1.Soil and Fertilizer Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China; |
| |
| Abstract: | The polymerase chain reaction denaturing gradient gel electrophoresis(PCR DGGE) was used to investigate the community structure and species diversity of ammonia oxidizing archaea(AOA) during poultry species composting. The results show that the AOA community structure and species diversity at different stages of chicken manure composting are markedly changed, and the band b, which shows 96% similarity to the AOA HH-2(GU2258721) and band m, which shows 99% similarity to the uncultured Crenarchaeote NM-152(HQ8752251), represent the major AOA bacterial species during the composting. The bands c, b, f and i, and the bands m, k, l and n represent the species of the two populations of bacteria, respectively. Shannon Weiner index (H) and evenness index (EH) of the AOA colonies are different at different stages of the composting, and the following order is: day 30>day 5>day 25≈day 45>day 3≈day 12>day 1≈day 15 The redundancy analysis of the AOA colonies at different stages of the composting shows that the AOA colony evolution is all significantly affected by the composting temperature, whole nitrogen, ammonia nitrogen and nitrate nitrogen (P<005), while pH is not affected. The AOA community structure is changed markedly on days 1, 5, 15, 30 and 45 of the composting. These results indicate that several parameters could control AOA community structure during composting of chicken manure. |
| |
| Keywords: | aerobic compost ammonia-oxidizing archaea(AOA) PCR-DGGE (denaturing gradient gelelectrophoresis,DGGE) community structure |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《植物营养与肥料学报》浏览原始摘要信息 |
|
点击此处可从《植物营养与肥料学报》下载全文 |
|
