| 北京黑猪PPARD基因G32E多态位点与脂肪沉积相关性状的关联分析 |
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| 引用本文： | 梁晶,王红卫,程利安,张龙超,王立刚,刘欣,石国华,杨洪平,刘先策,施辉毕,张跃博,张恬,王立贤. 北京黑猪PPARD基因G32E多态位点与脂肪沉积相关性状的关联分析[J]. 中国畜牧兽医, 2015, 42(7): 1793-1799. DOI: 10.16431/j.cnki.1671-7236.2015.07.025 |
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| 作者姓名： | 梁晶 王红卫 程利安 张龙超 王立刚 刘欣 石国华 杨洪平 刘先策 施辉毕 张跃博 张恬 王立贤 |
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| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;2. 北京黑六牧业科技有限公司, 北京 102211 |
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| 基金项目： | 北京市科技计划(Z121100001512004);国家自然科学基金(31201781);中国农业科学院基本科研业务专项(2012ZL069、2014ZL006) |
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| 摘 要： | 试验旨在研究猪过氧化物酶体增殖物激活受体δ(PPARD)基因第1外显子上的错义突变位点G32E(g.61399G>A)多态性与北京黑猪脂肪沉积相关性状的关联。利用Sequenom质谱检测平台检测北京黑猪及多个中外猪种群体G32E位点的基因型,并分析该位点多态性与北京黑猪背膘厚及肌内脂肪的关联性。结果发现,在北京黑猪中,G32E位点GG基因型个体背膘厚性状极显著高于GA和AA基因型个体(P<0.01),与肌内脂肪含量的关联未达到显著水平(P>0.05)。在梅山猪和二花脸猪中,主要以A等位基因为主;而在中国地方猪种民猪、莱芜黑猪、丫杈猪及国外商品猪种杜洛克猪、长白猪和大白猪中,G等位基因频率高。初步认为PPARD基因G32E位点多态性对背膘厚性状有显著影响,对肌内脂肪含量性状没有影响。
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| 关 键 词： | 猪 PPARD基因 DNA多态性 背膘厚 |
| 收稿时间： | 2014-12-23 |
Association Study on PPARD Gene G32E Polymorphic Site with Fat Deposit Related Traits in Beijing Black Pigs |
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| LIANG Jing,WANG Hong-wei,CHENG Li-an,ZHANG Long-chao,WANG Li-gang,LIU Xin,SHI Guo-hua,YANG Hong-ping,LIU Xian-ce,SHI Hui-bi,ZHANG Yue-bo,ZHANG Tian,WANG Li-xian. Association Study on PPARD Gene G32E Polymorphic Site with Fat Deposit Related Traits in Beijing Black Pigs[J]. China Animal Husbandry & Veterinary Medicine, 2015, 42(7): 1793-1799. DOI: 10.16431/j.cnki.1671-7236.2015.07.025 |
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| Authors: | LIANG Jing WANG Hong-wei CHENG Li-an ZHANG Long-chao WANG Li-gang LIU Xin SHI Guo-hua YANG Hong-ping LIU Xian-ce SHI Hui-bi ZHANG Yue-bo ZHANG Tian WANG Li-xian |
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| Affiliation: | 1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. Beijing Heiliu Animal Husbandry Science and Technology Co., Ltd., Beijing 102211, China |
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| Abstract: | The objective of this study was to analyze the association of a missense mutation G32E in the first exon of peroxisome proliferator-activated receptor delta (PPARD) gene with fat deposit related traits in Beijing Black pigs.Detected the genotype at G32E site in Beijing Black pig population and several Chinese and foreign pig populations using Sequenom mass spectrometry,and analyzed polymorphism with backfat thickness and IMF in Beijing Black pig population.The results indicated that backfat thickness of individuals with genotype GG was extremely significant higher than those individuals with genotypes GA and AA (P<0.01) in Beijing Black pig population,but no significant different of IMF was found (P>0.05).Allele A was high in Chinese local pigs Meishan and Erhualian.But in Minzhu,Laiwu,Yacha,and foreign commercial pigs Duroc,Landrace,Large White,mainly was allele G.The result suggested that polymorphism of PPARD gene G32E site had significant impact on backfat thickness,and no effect on IMF trait. |
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| Keywords: | pig PPARD gene DNA polymorphism backfat thickness |
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