Effects of Recombinant E. coli Expressing Heat-stable Enterotoxin on Intestinal Morphology and Antioxidant Capacity in Seven Days old Piglets |
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| Authors: | LV Yang ZHANG Lin LI Xue-ni ZHAO Di WU Tao |
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| Affiliation: | 1. Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023, China;2. Freshwater Fish Germ Plasm Quality Supervision and Testing Center, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430070, China |
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| Abstract: | This experiment was conducted to investigate the effects of recombinant E.coli expressing heat-stable enterotoxin(STa) on intestinal absorption and barrier function, intestinal morphology and antioxidant capacity of 7 days old piglets. Twenty-four 7 days old piglets were allotted to four treatments:control group (artificial milk), STa group (artificial milk +2×109 CFU E.coli LMG194-pBAD-STa), LMG194 group (artificial milk +2×109 CFU E.coli LMG194), and K88 group (artificial milk +2×109 CFU E.coli K88).The pigs were treated with E.coli on the 5th day and slaughtered on the 7th day. The results showed that, compared with the control group, villus height in jejunum, ileum and duodenum, crypt depth and villus height/crypt depth in duodenum, and small intestine villi surface area were significantly decreased in STa group (P<0.05). The activities of catalase (CAT) in ileum and colon, total nitric oxide synthase (TNOS) in serum, ileum, jejunum and colon, inducible nitric oxide synthase (iNOS) in colon were significantly decreased in STa group (P<0.05). STa group also had a higher levels of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in serum (P<0.05). These results suggested that recombinant E.coli expressing STa could lead to intestinal injury and oxidative stress of 7 days old piglets. |
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| Keywords: | heat-stable enterotoxin(STa) recombinant E.coli intestinal morphology antioxidant |
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