Establishment of Digital RT-PCR Assay for Detection of Swine Influenza Virus H3N2 Subtype |
| |
| Authors: | TANG Lian-fei YU Si-yu ZHOU Yu |
| |
| Affiliation: | 1. Inspection & Quarantine Technology Center of Hunan Entry-Exit Inspection & Quarantine Bureau, Changsha 410004, China;2. Huizhou Entry-Exit Inspection & Quarantine Bureau, Huizhou 516006, China |
| |
| Abstract: | A new assay for the detection of swine influenza virus (SIV) was developed with a novel nucleic acid probe——Molecular beacon in this study. The specific primers and molecular beacon probes were designed according to the conserved region of H3 and N2 genes of SIV H3N2 subtype. A digital RT-PCR assay was developed for detection of SIV H3N2 subtype. The results showed that SIV H3N2 subtype could be identified simultaneously on this microarry with high sensitivity and reproducibility,which could reach to 106 dilute viruse. The conclusion was that the digital RT-PCR method could analyze quantitatively the RNA templates.On the identification of H3N2 SIV,the digital RT-PCR method was much more scientific than Real-time quantitative PCR method. |
| |
| Keywords: | swine influenza virus H3N2 subtype molecular beacon probe digital RT-PCR Real-time quantitative PCR sensitivity specificity repeatability |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
|
点击此处可从《中国畜牧兽医》下载全文 |
