Isolation,Identification and Analysis of Drug Resistance of Salmonella Pullorum |
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| Authors: | LIU Zhi-ke YANG Ning-ning XU Jin-feng XU Ming-guo JING Ming-long WU Wen-xing CAO Xu-dong REN Yan SHI Feng CHEN Chuang-fu |
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| Affiliation: | 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;2. College of Life Sciences, Shihezi University, Shihezi 832003, China;3. School of Medicine, Shihezi University, Shihezi 832002, China |
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| Abstract: | In order to investigate the epidemic situation and drug resistance of major epidemic Salmonella Pullorum in some areas of Xinjiang, 750 chickens with disease history were detected using the methods of plate agglutination, and a total of 78 cases were diagnosed infection with Salmonella Pullorumin by using Salmonella identification medium, histopathological observation, PCR and drug sensitivity test.The results showed that 69 of 78 cases suspect Salmonella Pullorum were isolated, and prove the biochemical characteristics and pathologic characteristics of Salmonella Pullorum, the invA gene was amplified successfully by PCR,the isolation rate was 9.2% and the serotype was diagnosed as D1. The isolates were the most resistant to vancomycin and neomycin, and Salmonella Pullorum resistant rate was 100.0% and 81.2%, respectively. Drug sensitive test results showed that the sensitive rate of the bacteria were more than 60.0% to ceftriaxone,clavulanate and polymyxin. This study provided reference for farmers and veterinary workers, which indicated it could be used for the purification of Salmonella Pullorum and clinical medication. |
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| Keywords: | Salmonella Pullorum isolation and identification histopathology PCR drug sensitivity test |
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