Horizontal gene transfer of a syp homolog contributes to the virulence of Burkholderia glumae |
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| Authors: | WANG Sai WANG Pei-hong NIE Wen-han CUI Zhou-qi LI Hong-yu WU Yan Ayizekeranmu YIMING FU Luo-yi Iftikhar AHMAD CHEN Gong-you ZHU Bo |
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| Affiliation: | 1 School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture of the Ministry of Agriculture and Rural Affairs, Shanghai 200240, P.R.China2 Department of Plant Pathology & Ecology, the Connecticut Agricultural Experiment Station, New Haven 06504, CT, USA3 Department of Environmental Sciences, COMSATS University Islamabad, Vehari-Campus, Vehari 61100, Pakistan |
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| Abstract: | Horizontal gene transfer (HGT) has been proved a major driving force in prokaryotic evolution. However, the molecular functions of these transferred genes in pathogenic bacteria especially plant pathogenic bacteria are still not fully investigated. In this study, the whole-genome in silico analysis was performed and found a syringopeptin synthetase (syp) homolog in Burkholderia glumae, which can cause bacterial panicle blight in rice, was predicted to be horizontally transferred from Pseudomonas ancestor with solid confidence by phylogenetic analysis. The comprehensive molecular experiments were performed to study the potential role of this gene in B. glumae. Inoculation of rice panicles with the syp mutant resulted in 60% lower disease index compared with the wild type (WT) parent strain, suggesting the requirement of syp for the full virulence of B. glumae. Chromatography analysis of exudates from B. glumae showed suppression of synthesis of metabolites analogous to syringopeptin in the mutants. All these data raise the possibility of HGT phenomenon in shaping the virulence and adaptation of B. glumae over evolutionary time. |
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| Keywords: | horizontal gene transfer burkholderia glumae syringopeptin synthetase |
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