| 杂交构树根际固氮菌的分离与特性研究 |
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| 引用本文： | 张军,彭轶楠,郭琪,王沛雅,李鑫,杨晖. 杂交构树根际固氮菌的分离与特性研究[J]. 林业科学研究, 2021, 34(3): 187-193. DOI: 10.13275/j.cnki.lykxyj.2021.03.022 |
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| 作者姓名： | 张军 彭轶楠 郭琪 王沛雅 李鑫 杨晖 |
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| 作者单位： | 甘肃省科学院生物研究所，甘肃省微生物资源开发利用重点实验室，“特色微生物与植物资源创新”甘肃省国际科技合作基地，甘肃 兰州 730000 |
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| 基金项目： | 兰州市城关区科技计划;甘肃省科学院科技产业化项目 |
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| 摘 要： | 目的 获得杂交构树根际固氮菌菌株并明确其功能特性。 方法 以Ashby培养基为选择分离条件，从甘肃天水、兰州、张掖种植试验区的杂交构树根系及根际土壤中分离固氮菌，对固氮菌进行鉴定及系统发育分析，同时测定固氮酶活性、溶磷、分泌吲哚乙酸和抑制植物病原菌的能力，并采用熵权法对菌株特性进行综合评价。 结果 从远根土和根表土中共分离出10株固氮菌，分属8个属，以假黄单胞菌属（Pseudoxanthomonas sp.）居多。各菌株均具有固氮酶活性，TS4和HP10的固氮酶活性均大于180 IU·L−1；仅TS4和HTZ2具有溶磷能力；有8株菌能够分泌吲哚乙酸，HTZ4的分泌能力最强，分泌量为44.62 µg·mL−1。所有菌株对灰霉菌（Botrytis cinerea Persoon）无拮抗作用；有7株菌分别对至少2种以上植物病原菌有抑制作用。HTZ4、HTZ3、HP5分别对茄链格孢（Alternaria solani（Ellis et G. Martin) Sorauer）、尖孢镰刀菌（Fusarium oxysporum Schlecht）、腐皮镰孢菌（Fusarium solani (Martius) Sacco）有最强抑制，抑制率分别为50.00%、48.78%、47.37%。综合性能较强的菌株为TS4、HP5、ZY9、HTZ4和HTZ5。 结论 研究结果可为进一步开发综合性能优良的固氮菌株，研制杂交构树多功能固氮菌肥，提高西北半干旱地区杂交构树产量提供依据和指导。
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| 关 键 词： | 杂交构树 固氮 溶磷 分泌IAA 抑制病原菌 |
| 收稿时间： | 2021-01-11 |
Isolation and Investigation on Properties of Nitrogen-fixing Bacteria from Rhizosphere of Broussonetia papyrifera Hybrid |
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| ZHANG Jun,PENG Yi-nan,GUO Qi,WANG Pei-ya,LI Xin,YANG Hui. Isolation and Investigation on Properties of Nitrogen-fixing Bacteria from Rhizosphere of Broussonetia papyrifera Hybrid[J]. Forest Research, 2021, 34(3): 187-193. DOI: 10.13275/j.cnki.lykxyj.2021.03.022 |
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| Authors: | ZHANG Jun PENG Yi-nan GUO Qi WANG Pei-ya LI Xin YANG Hui |
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| Affiliation: | Institute of Biology, Gansu Academy of Sciences, Key Laboratory of Microbial Resources Exploitation and Application, Gansu International Science and Technology Cooperation Base of Microorganism and Plant Germplasm Resources & Genetic Improvement, Lanzhou 730000, Gansu, China |
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| Abstract: | Objective To obtain strains of nitrogen-fixing bacteria from Broussonetia papyrifera (Linnaeus) L’Héritier ex Ventenat hybrid and determine their functional characteristics. Method The Ashby medium was selected as separation condition to isolated nitrogen-fixing bacteria from the roots and rhizosphere soil of B. papyrifera hybrid in Tianshui, Lanzhou and Zhangye experimental areas of Gansu Province. In addition, identification and phylogenetic analysis were carried out on the isolated nitrogen-fixing bacteria. The nitrogenase activity, phosphorus solubility, properties of IAA-producing and inhibition of plant pathogenic fungi of each strain were determined at the same time. Entropy weight method was used for comprehensive evaluation of the features of the strains. Result A total of 10 nitrogen-fixing bacteria was isolated from the soil far from the rhizosphere and topsoil, which belonged to 8 genera, mostly Pseudoxanthomonas. Each strain showed nitrogenase activity, among which the nitrogenase activity of the strains TS4 and HP10 was greater than 180 IU·L−1. Only the strains TS4 and HTZ2 had certain phosphorus solubility. 8 strains could secrete IAA, among which the strain HTZ4 demonstrated the strongest secretion ability, with an IAA increment of 44.62 µg·mL−1. All strains showed no antagonistic effect on Botrytis cinerea Persoon, 7 strains showed inhibitory effects on at least two kinds of plant pathogens. The strains HTZ4, HTZ3, and HP5 showed the strongest inhibitory activities against Alternaria solani (Ellis et G. Martin) Sorauer, Fusarium oxysporum Schlecht and Fusarium solani (Martius) Sacco, respectively, with inhibition rates of 50.00%, 48.78% and 47.37%. To sum up, the strains with strong comprehensive properties were the strains TS4, HP5, ZY9, HTZ4 and HTZ5. Conclusion The results of this study can provide data and references for further developing nitrogen-fixing strains with excellent comprehensive performance, multifunctional nitrogen-fixing bacterial fertilizer for Broussonetia papyrifera hybrid, and promoting the yield of Broussonetia papyrifera hybrid in semi-arid area of Northwest China. |
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| Keywords: | Broussonetia papyrifera hybrid nitrogenase activity phosphorus solubility IAA-producing inhibiting plant pathogens |
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