| 水稻细胞质雄性不育花药和叶片中的活性氧代谢研究 |
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| 引用本文： | 刘 莉,蒋 苑,马 静,高志萍,陈国祥. 水稻细胞质雄性不育花药和叶片中的活性氧代谢研究[J]. 中国农学通报, 2016, 32(12): 6-12. DOI: 10.11924/j.issn.1000-6850.casb15100093 |
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| 作者姓名： | 刘 莉 蒋 苑 马 静 高志萍 陈国祥 |
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| 作者单位： | (南京师范大学生命科学学院植物所,南京 210023） |
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| 基金项目： | 国家自然科学基金“水稻剑叶叶绿体光能转化与碳同化协调运转的研究”(31271621)；江苏省自然科学青年基金“水稻细胞质雄性不育新种质‘盛世A’的基因克隆和功能分析”(BK20140916)；江苏省高校自然科学研究项目“杂交稻剑叶中脉中类似C4光合作用特性研究”(14KJB180011)。 |
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| 摘 要： | 为探究水稻雄性不育与活性氧代谢的内在关系，以水稻细胞质雄性不育系天丰A及保持系天丰B生殖生长期的花药和叶片为材料，对其O2-产生速率、H2O2和MDA含量以及抗氧化酶SOD、POD、CAT活性的变化进行测定。结果显示：生殖生长期间，不育系花药中O2-产生速率、H2O2和MDA含量总体高于保持系，抗氧化酶SOD、CAT活性总体低于保持系，而POD活性始终比保持系花药高；不育系与保持系叶片中仅表现出H2O2、MDA含量和CAT活性的差异。以上结果综合表明，不育系与可育系活性氧代谢的差异主要存在于花药中，花药发育过程中O2-、H2O2代谢失调和MDA过量积累以及保护酶活性下降或功能转变可能是导致雄性不育的重要因素。
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| 关 键 词： | 生物炭 生物炭 水稻(Oryza sativa) 发芽率 幼苗发育 |
| 收稿时间： | 2015-10-22 |
| 修稿时间： | 2015-11-07 |
Metabolism of Reactive Oxygen Species in Anthers and Leaves of Cytoplasmic Male-Sterile Rice |
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| Liu Li,Jiang Yuan,Ma Jing,Gao Zhiping and Chen Guoxiang. Metabolism of Reactive Oxygen Species in Anthers and Leaves of Cytoplasmic Male-Sterile Rice[J]. Chinese Agricultural Science Bulletin, 2016, 32(12): 6-12. DOI: 10.11924/j.issn.1000-6850.casb15100093 |
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| Authors: | Liu Li Jiang Yuan Ma Jing Gao Zhiping Chen Guoxiang |
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| Affiliation: | (College of Life Science, Nanjing Normal University, Nanjing 210023) |
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| Abstract: | In order to investigate the relationships between CMS and metabolism of reactive oxygen species, anthers and leaves of cytoplasmic male-sterile rice and its maintainer at reproductive stages were used as experimental materials to measure the production rate of O2-, contents of H2O2 and MDA, and activities of antioxidant enzymes (SOD, CAT and POD). The results showed that at reproductive stages, anthers of CMS line had higher production rate of O2-, contents of H2O2 and MDA, in comparison with the maintainer line. The activities of SOD and CAT were obsessively lower in CMS anthers, whereas POD activity of CMS anthers was higher than those of maintainer line. There were only differences in contents of H2O2 and MDA, and activity of CAT in leaves of male-sterile line and maintainer line. Based on the above results, the differences of the reactive oxygen metabolism between CMS and its maintainer were mainly apparent in anthers. In the process of anthers development, disordered metabolism of O2- and H2O2, excessive accumulation of MDA and decline of protective enzymes activities or function change of some protective enzymes may be the important reasons to make male sterility. |
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| Keywords: | rice cytoplasmic male sterile reactive oxygen metabolism |
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