| 油萝卜抗胞囊线虫基因的染色体定位及其在油菜附加系中的稳定性 |
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| 引用本文： | 张绍松,Peterka H,Budahn H,Schrader O,李成云,李进斌. 油萝卜抗胞囊线虫基因的染色体定位及其在油菜附加系中的稳定性[J]. 中国农业科学, 2008, 41(1): 93-101. DOI: 10.3864/j.issn.0578-1752.2008.01.012 |
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| 作者姓名： | 张绍松 Peterka H Budahn H Schrader O 李成云 李进斌 |
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| 作者单位： | 云南省农业生物技术重点实验室/农业部南方高原农业生物技术重点实验室 |
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| 基金项目： | 农业部引进交流处和云南省科技厅国际合作项目 |
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| 摘 要： | 【目的】油萝卜（2n=18，9条染色体分别被称作a～i）具有抗甜菜胞囊线虫的基因。探索油萝卜携带的抗甜菜胞囊线虫基因所在的染色体和外源的油萝卜染色体在油菜中的遗传稳定性。【方法】9个油菜附加系进行自花受粉，分别获得附加系的自交F2代。利用筛选的分子标记检测和原位杂交（FISH）检测附加染色体在F2代中的遗传稳定性；通过对9个油菜附加系F2代接种甜菜胞囊线虫的2龄幼虫，鉴定携带抗甜菜胞囊线虫基因所在的染色体。【结果】9个附加系自交F2代中的附加染色体遗传表现不一，5个油菜附加系的F2代中均有2条附加染色体,能稳定遗传；4个附加系的自交后代中附加染色体的遗传表现不稳定，部分后代中无附加染色体。FISH检测9个附加系自交F2代中的附加染色体表明：与分子标记检测的结果相符，并且4个不稳定的附加系中，有的自交后代虽然有附加染色体，但有1或2条。接种甜菜胞囊线虫测定结果表明，含有油萝卜染色体DD的油菜附加系对甜菜胞囊线虫的抗性水平与附加染色体的供体油萝卜相当，而其它8个附加系的自交后代未表现出抗性，与受体油菜相近。【结论】油萝卜的9条染色体分别附加到油菜中后，其遗传稳定性因附加的染色体而异，有的能稳定遗传，有的在自交后代中丢失1或2条；抗甜菜胞囊线虫的基因在d染色体上。
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| 关 键 词： | 油萝卜 抗性基因 胞囊线虫 染色体 定位 油菜附加系 稳定性 |
| 收稿时间： | 2006-06-17 |
| 修稿时间： | 2007-09-13 |
Chromosomal Localization of Resistance Gene in Radish Against Beet Cyst Nematode and the Stability of Additional Oil Radish Chromosomes in Rape-Radish Addition Lines |
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| ZHANG Shao-song,Peterka H,Budahn H,Schrader O,LI Cheng-yun,LI Jin-bin. Chromosomal Localization of Resistance Gene in Radish Against Beet Cyst Nematode and the Stability of Additional Oil Radish Chromosomes in Rape-Radish Addition Lines[J]. Scientia Agricultura Sinica, 2008, 41(1): 93-101. DOI: 10.3864/j.issn.0578-1752.2008.01.012 |
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| Authors: | ZHANG Shao-song Peterka H Budahn H Schrader O LI Cheng-yun LI Jin-bin |
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| Abstract: | Oil radish(2n=18,nine chromosomes were named as a-i) carry the resistance gene against the beet cyst nematode(BCN) Heterodera schachtii. Additional chromosome lines of rape for each segregated a-i were obtained.【Objective】Study on which chromosome of oil radish carry the resistance gene against BCN, and the stability of oil radish chromosomes in rape-radish addition lines. 【Method】Self-pollinated progeny F2 of nine rape-radish chromosome addition lines were tested by specific chromosomal molecular markers and fluorescence in situ hybridization (FISH). Those F2 plants were cultured in box and inoculated J2 larvae suspension of Heterodera schachtii. Then the number of cysts on plant roots were investigated 42 days after inoculation.【Result】They showed that the stability of additional oil radish chromosomes in rape is different. There were double additional chromosomes in each tested plant of rape-radish addition lines a, d, e, g, or i. In the rest addition lines, some plants carried additional radish chromosomes, however, some plants have not additional chromosome. Further experiment by FISH confirmed that the stable lines carried 2 additional chromosomes in each tested plant and the unstable lines have 2,1 or 0 additional chromosome(s) in different tested plants. After inoculated J2 larvae suspension of BCN, it shows that the plants of the DD addition line resistant to the BCN, but the other lines were susceptible. 【Conclusion】The hereditary stability of additional chromosomes in rape depend on different chromosomes of oil radish. And the resistance gene was located on “d ” chromosome of oil radish. |
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| Keywords: | Oil radish Resistance gene Cyst nematode Rape-radish addition lines Chromosome Localization Stability |
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