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| 抑制USP7活性对鸡抗沙门菌感染的影响 | |
| 引用本文： | 张娜,王菲,葛锡民,赵桂苹,文杰,李庆贺. 抑制USP7活性对鸡抗沙门菌感染的影响[J]. 畜牧兽医学报, 2022, 53(7): 2396-2402. DOI: 10.11843/j.issn.0366-6964.2022.07.036 |
| 作者姓名： | 张娜 王菲 葛锡民 赵桂苹 文杰 李庆贺 |
| 作者单位： | 1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;2. 西北农林科技大学动物科技学院, 杨凌 712100 |
| 基金项目： | 国家重点研发计划政府间国际科技创新合作重点专项(2018YFE0128000)； |
| 摘 要： | 本研究旨在阐明去泛素酶USP7对雏鸡抵抗鼠伤寒沙门菌感染的影响。选用3日龄的白来航鸡40只，随机分为2组，每组20只，隔天腹腔注射USP7抑制剂P5091或对照溶剂，连续注射5次，待最后一次注射结束后第4天，对雏鸡人工口服感染鼠伤寒沙门菌，感染24 h后，采集样品，RT-qPCR检测IL-1β、IL-8、TNFα以及NF-κB的mRNA表达，平板涂布法检测雏鸡肝载菌量，H&E染色观察肝和肺的炎症状况。结果表明，P5091抑制USP7活性后，转录因子NF-κB mRNA水平显著降低，并且下游细胞因子IL-1β、IL-8以及TNF-α的表达均下调，雏鸡载菌量下降，H&E染色显示肝与肺炎症反应加剧。综上表明，USP7可能在机体抗沙门菌感染方面起重要作用。 |
| 关 键 词： | USP7 鼠伤寒沙门菌 免疫反应 NF-κB 细胞因子 |
| 收稿时间： | 2021-11-11 |
Correlation between the Expression Level of USP7 and the Immune Response to Salmonella Infection in Chickens |
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| ZHANG Na,WANG Fei,GE Ximin,ZHAO Guiping,WEN Jie,LI Qinghe. Correlation between the Expression Level of USP7 and the Immune Response to Salmonella Infection in Chickens[J]. Chinese Journal of Animal and Veterinary Sciences, 2022, 53(7): 2396-2402. DOI: 10.11843/j.issn.0366-6964.2022.07.036 | |
| Authors: | ZHANG Na WANG Fei GE Ximin ZHAO Guiping WEN Jie LI Qinghe |
| Affiliation: | 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;2. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China |
| Abstract: | This research aimed to clarify the effect of ubiquitin-specific protease (USP7) on the resistance of chickens to Salmonella Typhimurium infection. Forty 3-day-old White Leghorns were randomly divided into two groups, and respectively intraperitoneal injected with the USP7 inhibitor P5091 and control solvent every other day for 5 consecutive injections (n=20 in each group). Four days after the last injection, they were artificially orally infected with S.Typhimurium. Samples were collected 24 hours after infection. The mRNA abundances of IL-1β, IL-8, TNF-α and NF-κB were examined by RT-qPCR. The bacteria load in the liver of chickens was measured by the plate coating method. Liver and lung inflammation was checked by H&E staining. Inhibition of the USP7 activity by P5091 treatment significantly downregulated the mRNA level of NF-κB, and its downstream cytokines IL-1β, IL-8 and TNF-α. The bacterial load in the liver of chickens decreased. H&E staining showed aggravated inflammation in the liver and lung in USP7 inhibited chickens. USP7 might have critical role in chicken to resist Salmonella infection. |
| Keywords: | USP7 Salmonella immune response NF-κB cytokines |
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