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| 银杏营养贮藏蛋白质的分离鉴定 | |
| 引用本文： | 彭方仁,郭红彦,吴青霞. 银杏营养贮藏蛋白质的分离鉴定[J]. 林业科学, 2010, 46(3): 52-55. DOI: 10.11707/j.1001-7488.20100308 |
| 作者姓名： | 彭方仁 郭红彦 吴青霞 |
| 作者单位： | 南京林业大学森林资源与环境学院南京 210037 |
| 基金项目： | 国家自然科学基金项目(30872055);;教育部高校博士点基金(20070298008) |
| 摘 要： | 采用光学和电子显微镜技术、SDS-PAGE技术、间接免疫酶标光镜定位和胶体金免疫电镜定位技术对银杏枝条中的营养贮藏蛋白质（VSPs）进行分离鉴定,并对其细胞学特性及动态变化规律进行初步研究。研究结果：运用制备型SDS-聚丙烯酰胺凝胶电泳分离并制备银杏32和36 ku 2种营养贮藏蛋白质抗原，通过免疫新西兰大白兔成功获得银杏32和36 ku 2种营养贮藏蛋白质的多克隆抗体。银杏VSPs主要分布于落叶期当年生枝条的次生韧皮部细胞中，呈现出团块状、颗粒状和均一状3种形态。在新梢生长期，积累在枝条中的VSPs逐渐降解消失，表现出明显的季节变化 |
| 关 键 词： | 银杏 营养贮藏蛋白质 分离鉴定 免疫标记分析 |
| 收稿时间： | 2008-11-21 |
Isolation and Identification of Vegetative Storage Protein of Ginkgo biloba |
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| Peng Fangren Guo Hongyan Wu Qingxia. Isolation and Identification of Vegetative Storage Protein of Ginkgo biloba[J]. Scientia Silvae Sinicae, 2010, 46(3): 52-55. DOI: 10.11707/j.1001-7488.20100308 | |
| Authors: | Peng Fangren Guo Hongyan Wu Qingxia |
| Affiliation: | College of Forest Resources and Environment, Nanjing Forestry UniversityNanjing 210037 |
| Abstract: | The vegetative storage proteins(VSPs) in branches of Ginkgo biloba were isolated and identified by using light-and electron-microscope,SDS-PAGE,immunoenzyme optical-microscopy localization and immunogold electron-microscopy localization.The cytological characteristic and dynamic changes of VSPs were preliminarily studied.The results were as follows:The antigens of vegetative storage protein of 32 and 36 ku were separated and prepared by using SDS-polyacrylamide gel electrophoresis,antibodies of vegetative s... |
| Keywords: | Ginkgo biloba vegetative storage protein (VSPs) isolation and identification immuno-labeling analyzing |
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