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| 中国松树枯梢病菌遗传多态性的RAPD分析 | |
| 引用本文： | 吴小芹,黄敏仁,尹佟明. 中国松树枯梢病菌遗传多态性的RAPD分析[J]. 林业科学, 2000, 36(4): 32-38. DOI: 10.11707/j.1001-7488.20000406 |
| 作者姓名： | 吴小芹 黄敏仁 尹佟明 |
| 作者单位： | 南京林业大学,南京210037 |
| 基金项目： | 国家“九五”攻关项目!( 95 0 4 0 1) |
| 摘 要： | 运用随机扩增多态DNA(RAPD)技术对发生于我国13省区16种松树和其它2种针叶树上的松枯梢病菌(Sphaeropsis sapinea)的55个菌株进行基因组DNA多态性分析。用17个随机引物经PCR扩增共得到200个RAPD标记,其中98.5%具有多态性。UPGMA聚类分析确定了供试菌株间的亲缘关系,将55个菌株分为3个类群。各菌株间的差异与其寄主种类无明显关系,与其地理来源在某些类群间有一定联系,但在大多数菌株间相关趋势不明显。 |
| 关 键 词： | 松树枯梢病 Sphaeropsis sapinea RAPD标记 遗传多样性 |
| 收稿时间： | 1999-11-04 |
THE USE OF RAPD TO DETECT THE GENETIC VARIATION OF SPHAEROPSIS SAPINEA IN CHINA |
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| Wu Xiaoqin,Huang Minren,Yin Tongmin. THE USE OF RAPD TO DETECT THE GENETIC VARIATION OF SPHAEROPSIS SAPINEA IN CHINA[J]. Scientia Silvae Sinicae, 2000, 36(4): 32-38. DOI: 10.11707/j.1001-7488.20000406 | |
| Authors: | Wu Xiaoqin Huang Minren Yin Tongmin |
| Affiliation: | Nanjing Forestry University Nanjing 210037 |
| Abstract: | Genomic DNA from 55 isolates of Sphaeropsis sapinea collected on 16 pines and other 2 conifers from 13 provinces in China was studied using random amplified polymorphic DNA markers(RAPDs). The seventeen random primers produced 200 scorable DNA fragments. One hundred ninety seven fragments were polymorphic. The dendrogram generated by UPGMA divided all isolates into three groups. Cluster analysis showed that differences between isolates were not closely related to the host species and that there were complex relations between the isolate differences and geographic sources. |
| Keywords: | Sphaeropsis sapinea RAPD marks Genetic diversity |
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