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1株临床高致病性猪繁殖与呼吸综合征病毒的分离与鉴定
引用本文:王兴龙,柏坤桃,李玉峰,唐波,蒋文明,姜平.1株临床高致病性猪繁殖与呼吸综合征病毒的分离与鉴定[J].畜牧与兽医,2007,39(8):12-16.
作者姓名:王兴龙  柏坤桃  李玉峰  唐波  蒋文明  姜平
作者单位:1. 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095
2. 江苏省高邮市畜牧兽医站,江苏,高邮,225600
基金项目:致谢:本次试验得到江苏省高邮市畜牧兽医站的大力支持,在此表示感谢.
摘    要:本研究从江苏省某猪场患有明显呼吸道临床症状和高热死亡的仔猪肺脏组织中分离到一株病毒,经病毒生物学特性测定、血清学试验、病毒基因鉴定,确定为美洲型猪繁殖与呼吸综合征病毒(PRRSV)。人工猪体感染发病试验表明,该分离株可以引起商品仔猪出现明显临床症状和死亡,证实我国已经出现临床高致病性PRRSV毒株。

关 键 词:猪繁殖与呼吸综合征病毒  分离  致病性
文章编号:0529-5130(2007)08-0012-05
修稿时间:2007-04-05

Isolation and identification of a very highly pathogenic strain of porcine reproductive and respiratory syndrome virus
WANG Xing-long,BO Kun-tao,LI Yu-feng,TANG Bo,JIANG Wen-ming,JIANG Ping.Isolation and identification of a very highly pathogenic strain of porcine reproductive and respiratory syndrome virus[J].Animal Husbandry & Veterinary Medicine,2007,39(8):12-16.
Authors:WANG Xing-long  BO Kun-tao  LI Yu-feng  TANG Bo  JIANG Wen-ming  JIANG Ping
Institution:1. Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing 210095, China ; 2. Gaoyou Husbandry and Veterinary Station in Jiangsu Province, Gaoyou 225600, China
Abstract:One strain of virus was isolated from lung tissue of piglets died of obvious respiratory syndrome and high fever on a farm of Jiangsu province. The sensitivity of the virus to pH and temperature was determined. It was identified as a strain of American type PRRSV by serological test and gene sequencing. The result of animal test showed that the virus could cause apparent clinical syndrome and death of commercial piglets. This study confirmed the presence of very highly pathogenic PRRSV in China.
Keywords:PPRSV  isolation  pathogenicity
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