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金堂黑山羊LHR基因片段的克隆及序列分析
引用本文:张雪琴,刘丽,曹冶,赵素君,钟金城.金堂黑山羊LHR基因片段的克隆及序列分析[J].畜牧与兽医,2009,41(5).
作者姓名:张雪琴  刘丽  曹冶  赵素君  钟金城
作者单位:1. 西南民族大学生命科学与技术学院,四川,成都,610041
2. 四川省畜牧科学研究院,四川,成都,610066;四川大学生命科学院,四川,成都,610064
基金项目:四川省应用基础研究项目,四川省科技攻关项目 
摘    要:采用RT-PCR法从刚断奶健康金堂黑山羊卵巢组织中扩增出促黄体素受体(LHR)基因部分序列,序列长度分别为1799和1724个碱基。2个序列与牛相比,中间都缺失266 bp;与猪变体B有相同的终止密码,初步确定2个序列为山羊LHR的2个变体。2个序列蛋白没有7个跨膜结构,但有N连接糖基化位点。结果表明:金堂黑山羊LHR RNA中存在一种替代剪接机制,采用不同的相位导致读码框的终止。

关 键 词:金堂黑山羊  促黄体素受体基因  克隆

Cloning and sequence analysis on LHR gene fragments in the Jintang black goat
ZHANG Xue-qin,LIU Li,CAO Ye,ZHAO Su-jun,ZHONG Jin-cheng.Cloning and sequence analysis on LHR gene fragments in the Jintang black goat[J].Animal Husbandry & Veterinary Medicine,2009,41(5).
Authors:ZHANG Xue-qin  LIU Li  CAO Ye  ZHAO Su-jun  ZHONG Jin-cheng
Abstract:Two fragments of 1 799 bp and 1 724 bp from black goat luteinizing hormone receptor(LHR) gene were amplified from ovarian tissue of healthy female Jintang black goat by RT-PCR.The comparison on the gene nucleotide sequences between the goat and Bos taurus showed that,266 bp deletions in coding regions were both detected.They had the same termination codon as procine B variat.It is assumed the sequences were variants of LHR gene from black goat.There were no 7 transmembrance regions of LHR in Jintang black goat.However,there were six N-glycosylation sites in Jintang black goat.These results suggested that the LHR in the Jintang black goat were in an alternative splicing mechanism,a different phase leaded to the termination of reading frame.
Keywords:Jintang black goat  luteinizing hormone receptor gene  cloning
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