| 一株传染性法氏囊病病毒强毒株的分离与鉴定 |
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| 引用本文： | 刘维婷,廖梦,曹瑞兵.一株传染性法氏囊病病毒强毒株的分离与鉴定[J].畜牧与兽医,2012,44(5):10-13. |
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| 作者姓名： | 刘维婷 廖梦 曹瑞兵 |
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| 作者单位： | 南京农业大学动物医学院,江苏南京,210095 |
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| 摘 要： | 鸡传染性法氏囊病是由传染性法氏囊病病毒引起的一种急性传染病。本研究从江苏某疑似发生传染性法氏囊病的鸡场采集病料,通过观察临床症状、病理变化、RT-PCR检测、基因测序、SPF鸡胚接种、琼脂扩散试验和雏鸡攻毒等试验,证实了该鸡群发生了传染性法氏囊病,且分离到一株传染性法氏囊病病毒(JSXY株),该病毒有较强的致病力,VP4基因比较分析发现其与变异株亲缘关系最近,序列同源性为95%。本研究为江苏地区传染性法氏囊病的防治提供了有益的参考。
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| 关 键 词： | 传染性法氏囊病病毒 分离 鉴定 毒力 |
Isolation and identification of a virulent strain of infectious bursal disease virus |
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| LIU Wei-ting
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LIAO meng
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CAO Rui-bing.Isolation and identification of a virulent strain of infectious bursal disease virus[J].Animal Husbandry & Veterinary Medicine,2012,44(5):10-13. |
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| Authors: | LIU Wei-ting LIAO meng CAO Rui-bing |
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| Institution: | *(College of Veterinary Medicine,Nanjing Agriculutre University,Nanjing 210095,China) |
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| Abstract: | Infectious bursal disease(IBD),caused by infectious bursal disease virus(IBDV),is an important infectious disease.In the present study,the samples from a suspected chicken farm in Jiangsu province were detected by RT-PCR using IBDV vp4 specific primers,combined with the clinical symptoms,pathological changes,SPF embryo inoculation test and agar diffusion test.IBDV infection was confirmed in this farm.According to the results of artificial challenge test and the VP4 gene sequence analysis,the IBDV isolate is a virulent strain with VP4 gene variation.The result provides a useful reference for the prevention and control of IBD in Jiangsu province. |
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| Keywords: | infectious bursal disease virus isolation identification virulence |
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