| 动物产品及饲料中牛源和羊源性成分三重荧光PCR检测方法的建立 |
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| 引用本文： | 赵冉,蔡振鸿,陈永锋.动物产品及饲料中牛源和羊源性成分三重荧光PCR检测方法的建立[J].畜牧与兽医,2012,44(7):18-22. |
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| 作者姓名： | 赵冉 蔡振鸿 陈永锋 |
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| 作者单位： | 1. 厦门市农产品质量安全检验测试中心,福建厦门,361012
2. 新奥(厦门)农牧发展有限公司,福建厦门,361000 |
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| 摘 要： | 为鉴定和区分饲料及动物产品中牛、山羊、绵羊源性成分,根据线粒体DNA(mitochondrial DNA,mtDNA)种间保守序列,设计合成了3对特异性引物与TaqMan探针,通过对荧光PCR反应体系和反应条件的优化筛选,建立了三重荧光PCR方法,在同一个荧光PCR反应中完成3种动物源性成分的检测。用该方法对16种不同源性的动物DNA进行检测,结果表明能特异地鉴别检测出牛、山羊和绵羊源性成分,且敏感性比现行国标PCR法高100倍。该方法适用于饲料、肉制品、奶制品等动物源性产品的检测。
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| 关 键 词： | 线粒体DNA 荧光PCR 牛、山羊和绵羊源性成分 |
Establishment of multiplex fluorescent PCR assay for the detection of bovine,goat and sheep- derived materials in animal products and feeding stuffs |
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| ZHAO Ran
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CAI Zhen-hong
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CHEN Yong-feng.Establishment of multiplex fluorescent PCR assay for the detection of bovine,goat and sheep- derived materials in animal products and feeding stuffs[J].Animal Husbandry & Veterinary Medicine,2012,44(7):18-22. |
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| Authors: | ZHAO Ran CAI Zhen-hong CHEN Yong-feng |
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| Institution: | 1(1.Supervision and Testing Center for Agricultural Product Quality(Xiamen),Xiamen 361012,China; 2.Singao Agribusiness Development Co Ltd,Xiamen 361000,China) |
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| Abstract: | In this study,a multiplex fluorescent PCR assay capable of detecting and differentiating bovine,goat and sheep-derived materials was developed using specific primers and TaqMan probes designed according to mitochondrial DNA gene sequences.The multiplex fluorescent PCR reaction system was optimized.The developed method could successfully detect bovine,goat and sheep-derived materials of 16 animals.The result showed that the real-time PCR can work accurately and efficiently,and can be 100 times as sensitive as conventional PCR of GB/T 20190-2006.Therefore,this method has the potential application for detection of bovine,goat and sheep-derived materials in feed,meat products,milk products,etc. |
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| Keywords: | mitochondrial DNA multiplex fluorescent PCR bovine and goat and sheep-derived materials |
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