猪抗病基因Mx1第14外显子多态性与免疫性能的相关分析

为分析辽宁种猪群体Mx1基因多态性与免疫性能之间的关系,提取猪耳组织DNA,采用PCR-RFLP技术分析荷包猪、大白猪的Mx1基因多态性,通过双抗体夹心酶联免疫分析(ELISA)方法对两个种群中IFN-α、IL-4、SIgA的水平进行分析,显示:Hin6Ⅰ酶切仅检测到荷包猪存在AA、AB、BB三种基因型,基因型频率为0.681 4、0.061 9、0.256 6;荷包猪群体免疫数据整体高于大白猪群体,且波动范围大。结果表明:荷包猪与大白猪Mx1基因频率存在差别,荷包猪群体免疫水平高于大白猪,且携带更多Mx1基因的抗病性基因型。本研究为辽宁种猪资源保存、品种选育和开发利用提供了科学依据。

Relationship between polymorphisms of the exon 14 in Mx1 genes and immune performance in pigs

In order to analyze the relationship between Mx1 gene polymorphisms and the immune performance in Liaoning pigs,we extracted DNA from pig ear tissues.By the PCR-RFLP method,the polymorphisms of Mx1 gene were detected in Hebao and Large White pigs.ELISA was used to measure the levels of IFN-α,IL-4 and SIgA in the two populations.The analysis results showed that Mx1 gene digested by restriction endonuclease Hin6I only in Hebao pigs was divided into three kinds of genotypes of AA,AB and BB,and the frequencies of genotypes were 0.6814,0.0619 and 0.2566,respectively.In addition,the ELISA data of Hebao pigs were higher than Large White as a whole,and the fluctuation range was large.The results showed that higher immunity and much more resistance genotypes of Mx1 exsisted in Hebao pigs than those in Large White.Our finding provided the scientific basis for the resource preservation,variety breeding and the development and utilization of Hebao pigs.