种公猪精液中猪瘟和蓝耳病病毒混合感染的快速检测

参考GenBank公布的猪蓝耳病病毒(PRRSV)VL2332株、LV株以及猪瘟兔化弱毒(CSFV)C株的基因序列,各设计合成了一对引物,建立了在相同PCR扩增条件下能同时检测PRRSV和CSFV的RT-PCR方法。对2003~2004年期间江苏、浙江、安徽、福建、上海等省市的17个大中型猪场送检的186份种公猪精液进行了检测,结果18份呈PRRSV阳性,24份呈CSFV阳性,其中有11份为PRRSV和CSFV的混合感染,约占送检精液样品的5.91%。试验结果表明,所建立的RT-PCR方法可用于精液中这2种野毒感染的快速鉴定和分子流行病学调查。

Rapid detection for porcine reproductive and respiratory syndrome virus(PRRSV) and classical swine fever virus(CSFV) mixedly infected in boar semen

Using two pairs of specific primers designed according to the relevant nucleotide sequences of porcine reproductive and respiratory syndrome virus (PRRSV) VL 2332 strain, Lv strain and classical swine fever virus (CSFV) C strain from GenBank, a RT-PCR method was established to detect the two viruses in the same PCR condition at the same time.186 boar semen samples from 17 pig farms in Jiangsu, Shanghai, Zhejiang, Anhui and Fujian were tested with this method. Among these samples 18 were PRRSV positive, 24 CSFV positive, 11 both PRRSV and CSFV positive which amounted to 5.91 percent of all samples. This study provides a more sensitive, specific and reliable method to detect PRRSV and CSFV in boar semen rapidly.