| 高效液相-串联质谱法测定畜禽肌肉中泰妙菌素残留 |
| |
| 引用本文： | 耿士伟,朱志谦,曲斌,蒋天梅,陆桂萍.高效液相-串联质谱法测定畜禽肌肉中泰妙菌素残留[J].畜牧与兽医,2012,44(8):21-24. |
| |
| 作者姓名： | 耿士伟 朱志谦 曲斌 蒋天梅 陆桂萍 |
| |
| 作者单位： | 江苏省畜产品质量检验测试中心,江苏南京,210036 |
| |
| 摘 要： | 建立了固相萃取-高效液相色谱-串联质谱法测定畜禽肉中泰妙菌素残留的分析方法。试样经磷酸盐缓冲液(pH=8.0)-乙酸乙酯混合提取,经水饱和正己烷除脂后,过MCX固相萃取小柱净化,采用LC-MS/MS电喷雾电离正离子扫描,多反应监测模式(MRM)检测,外标法定量。泰妙菌素在0.5～50μg/L的浓度范围内呈现良好的线性关系,相关系数r大于0.999。其检出限为0.25μg/kg,定量限为0.5μg/kg。
|
| 关 键 词： | 畜禽 泰妙菌素 残留 高效液相色谱-串联质谱法 |
Determination of tiamulin residues in the muscles of livestock and poultry by HPLC-MS/MS |
| |
| GENG Shi-wei
,
ZHU Zhi-qian
,
QU Bin
,
JIANG Tian-mei
,
LU Gui-ping.Determination of tiamulin residues in the muscles of livestock and poultry by HPLC-MS/MS[J].Animal Husbandry & Veterinary Medicine,2012,44(8):21-24. |
| |
| Authors: | GENG Shi-wei ZHU Zhi-qian QU Bin JIANG Tian-mei LU Gui-ping |
| |
| Institution: | (Jiangsu Quality Inspection and Testing Center for Animal Products,Nanjing 210036,China) |
| |
| Abstract: | A high performance liquid chromatography tan-dem mass spectrometry(HPLC-MS/MS) method was developed to determine the tiamulin residues in the muscles of livestock and poultry by solid phase extraction(SPE).The sample was extracted by phosphate buffer(pH8.0)-ethyl acetate mixture solvent.After fat was removed by water-saturated n-hexane,the sample was cleaned by MCX mode SPE.Tiamulin was detected by LC-MS/MS with MRM mode.It showed a good linearity in the ranges of 0.5μg/L to 50μg/L.The detection and quantification limits were 0.25μg/kg and 0.5μg/kg,respectively. |
| |
| Keywords: | livestock and poultry tiamulin residue HPLC-MS/MS |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
