雌性中华鳖输卵管精子储存结构的研究

应用光镜和电镜技术,系统观察雌性中华鳖输卵管精子储存情况,显示与精子储存有关组织结构与细胞形态。结果表明,精子储存在雌性中华鳖输卵管的蛋白分泌部后部至子宫部,但各段的组织结构及精子储存量存在一定差别。蛋白分泌部后部上皮较发达,由典型的高柱状纤毛细胞和分泌细胞构成,固有膜中腺体多为泡状腺。上皮和腺体中含有大量高密度的膜性分泌颗粒,此段只有少量的精子储存。峡部较窄且固有膜内无腺体,上皮排列紧密并呈迷路样迂回分布,上皮细胞内高电子密度分泌颗粒成团集中分布在核上方。峡部管腔中分布着大量的精子,靠近管腔的精子或精子头部嵌入上皮纤毛之间或顶端凹陷的胞质中,且精子嵌入部分的细胞结构保持完整。子宫前部形成垂直于管腔的储精小管(SST),子宫上皮及此段SST上皮的分泌颗粒电子密度不均,含高密度电子致密斑,腺体中的分泌颗粒也呈现不同的内部结构。子宫部及SST的管腔中储存有大量精子。这些区段复杂的细胞结构及分泌活动,可能在精子储存中发挥重要作用。

Oviductal sperm storage structure in the female soft-shelled turtle Trionyx sinensis

We investigated the structural features of sperm storage in the oviduct of female soft-shelled turtle Trionyx sinensis by light microscope and transmission electron microscope.The result showed that sperms were distributed from posterior uterine tube to uterus.However,the quantity of sperm and structure of each region showed marked difference.In posterior uterine tube,the epithelium of mucosa consisted of columnar secretory and ciliated cells.Ciliated cells only showed few secretory granules(SGs).Secretory cells showed more SGs in cytoplasm,and the size,shape,and electron density of these granules varied markedly.The tubular glands in lamina propria also contained various SGs similar to SGs of secretory cell in this region.The isthmus was a short,nonglandular region.The mucosa showed longitudinal grooves as in the tube and folds as labyrinthine.The SGs were electron-dense,mostly concentrated to the supranuclear region.Large numbers of sperm remained in aggregates in the lumen or embed into the epithelium in this region,the embedded portions of sperm generally appeared normal in structure.The anterior uterus showed to form sperm storage tubules(SSTs).The epithelium of uterus and SSTs showed branching SGs with irregular dense cores.The gland in this region also contained numerous membrane-bound SGs with various internal ultrastructure.Complex cell structures and secretory activity of these regions may be important for sperm storage.