| 革兰阴性菌外膜囊泡的研究进展 |
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| 引用本文： | 卞志标,李冰,勾红潮,杨君,臧莹安,李春玲.革兰阴性菌外膜囊泡的研究进展[J].畜牧与兽医,2020(5):136-142. |
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| 作者姓名： | 卞志标 李冰 勾红潮 杨君 臧莹安 李春玲 |
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| 作者单位： | 仲恺农业工程学院;广东省农业科学院动物卫生研究所/广东省畜禽疫病防治研究重点实验室/农业部兽用药物与诊断技术广东科学观测实验站;茂名市动物疫病预防控制中心 |
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| 基金项目： | 国家自然科学基金(31772776,31502107);广东省自然科学基金(2015A030313562、2017A030310074);广东省科技计划(2014A010107020);广州市科技计划(201607010380)。 |
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| 摘 要： | 外膜囊泡(outer membrane vesicles,OMVs)是革兰阴性(G^-)菌从细胞膜上脱落下来的囊泡,携带有细菌的大量组分,如外膜蛋白、脂多糖、脱氧核糖核酸等。由于OMVs无活性、不能复制且具有免疫原性,所以被认为是最具潜力的亚单位疫苗,同时OMVs对细菌本身具有多种作用,故OMVs成为研究的热点。本文从G^-菌OMVs的组成成分、形成机制、生物学功能、提取纯化方法及其开发应用前景5个方面综述其研究现状,为G^-菌的致病机制研究、OMVs的疫苗开发应用提供技术指导。
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| 关 键 词： | OMVs 形成机制 组分分析 生物学功能 |
Advances in research on outer membrane vesicles from gram-negative bacteria |
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| BIAN Zhibiao,LI Bing,GOU Hongchao,YANG Jun,ZANG Ying'an,LI Chunling.Advances in research on outer membrane vesicles from gram-negative bacteria[J].Animal Husbandry & Veterinary Medicine,2020(5):136-142. |
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| Authors: | BIAN Zhibiao LI Bing GOU Hongchao YANG Jun ZANG Ying'an LI Chunling |
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| Institution: | (Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Institute of Animal Heath,Guangdong Academy of Agricultural Sciences/Guangdong Province Key Laboratory of Livestock Disease Prevention,Guangzhou 510640,China;Maoming Animal Disease Prevention and Control Center,Maoming 525000,China) |
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| Abstract: | OMVs are the vesicles of G^-bacteria detached from the cell membrane,carrying a large number of components of bacteria,such as OMP,LPS,DNA,etc.Due to its inactivity,irreproducibility and immunogenicity,OMVs are considered to be the most promising subunit vaccine,in addition to their multiple effects on bacteria themselves;therefore OMVs have become a research hotspot.In this paper,the progress in research on OMVs is summarized from five aspects including their synthesis mechanism,extraction and purification methods,composition analysis,biological function and development and application prospect,which would provide technical guidance for the in-depth study of OMVs,the pathogenic mechanism of G^-bacteria,and the development and application of OMV vaccine. |
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| Keywords: | OMVs synthesis mechanism component analysis biological function |
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