以MTT比色法检测鸡脾淋巴细胞转化效果

本研究运用ＭＴＴ比色法以正交试验对鸡脾脏淋巴细胞转化的最佳条件（ｃｏｎＡ量、血清种类和浓度）进行了研究。结果表明，无论血清种类和浓度如何，低剂量ＣｏｎＡ（２．５～１０μｇ／ｍｌ）可以获得较好的转化效果。当ＣｏｎＡ的量确定时，低浓度鸡血清（０．５％～１．２５％）的转化效果优于高浓度（２％～３％）：５％犊牛血清优于任何浓度的鸡血清；无血清培养基优于血清培养基。其最佳组合为无血清ＲＰＭＩ１６４０培养基加ＣｏｎＡ２．５μｇ／ｍｌ。

MEASUREMENT OF CHICKEN SPLENIC LYMPHOCYTE ACTIVATION BY MTT COLORIMETRIC ASSAY

MTT colorimetric assay was used to measure the perfect conditions(ConA concentrations,kinds andconcentrations of serums)for chicken splenic lymphocyte activation in orthogonal experiment. It was found that lowdoes ConA(2.5～10μg/ml)with 0～3%chicken serum or 5%fetal calf serum could resulted in a good activation incontrast with high dose ones(20～60μg/ml ).Whcn the conccntration of ConA was determined,Iow concentrationchicken serum(0～1.25%)induced higher activation than that induced by high ones(2～3%).The activationrcsulted by fetal ealf serum,howerver,was better than that resulted by chicken scrum of any concentrations. Themore perfect conditions for activation were RPMI 1640 medium with 2.5μg/ml ConA.