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| 骨形态发生蛋白IB型受体(BMPR-IB)基因在蒙古羊卵巢和子宫组织中的表达分析 | |
| 引用本文： | 何小龙,刘永斌,王峰,田春英,达赖,荣威恒.骨形态发生蛋白IB型受体(BMPR-IB)基因在蒙古羊卵巢和子宫组织中的表达分析[J].畜牧与兽医,2010,42(8). |
| 作者姓名： | 何小龙 刘永斌 王峰 田春英 达赖 荣威恒 |
| 作者单位： | 1. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018 2. 内蒙古农牧业科学院,内蒙古,呼和浩特,010030 3. 内蒙古农业大学动物科学与医学学院,内蒙古,呼和浩特,010018;内蒙古农牧业科学院,内蒙古,呼和浩特,010030 |
| 基金项目： | 内蒙古农牧业科学院青年科技创新基金，国家科技攻关计划，国家现代肉羊产业技术体系建设专项 |
| 摘 要： | BMPR-IB基因是发现最早且对绵羊排卵率影响机理已经阐明的多胎主效基因,主要在与繁殖有关的组织和器官中表达,并且对绵羊排卵起重要的调控作用。本研究采用实时荧光定量PCR(Real-time Quantitative PCR,RQ-PCR)技术的相对定量方法对BMPR-IB基因在不同生理时期蒙古羊的卵巢和子宫组织进行了差异表达的研究。定量结果得到-βActin基因的扩增曲线回归方程为y=-3.358x+35.708,回归系数R2=0.990,BMPR-IB基因的扩增曲线回归方程为y=-2.119x+31.424,回归系数R2=0.992。以非发情期蒙古羊的卵巢组织定量结果为对照计算得到BMPR-IB基因在发情期蒙古羊的卵巢组织中表达量最高,在发情期的子宫组织中表达量最低,在发情期卵巢组织中的表达量是非发情期的2.65倍。 |
| 关 键 词： | 蒙古羊 BMPR-IB基因 差异表达 实时荧光定量PCR |
Expression of BMPR-IB gene in the ovarian and uterus tissues of Mongolian sheep |
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| HE Xiao-long,LIU Yong-bin,WANG Feng,TIAN Chun-ying,Da Lai,RONG Wei-heng.Expression of BMPR-IB gene in the ovarian and uterus tissues of Mongolian sheep[J].Animal Husbandry & Veterinary Medicine,2010,42(8). | |
| Authors: | HE Xiao-long LIU Yong-bin WANG Feng TIAN Chun-ying Da Lai RONG Wei-heng |
| Institution: | HE Xiao-long1,LIU Yong-bin2,WANG Feng2,TIAN Chun-ying2,Da Lai2,RONG Wei-heng1,2(1.College of Animal Science and Medicine,Inner Mongolian Agricultural University,Huhhot 010018,China,2.Inner Mongolia Academy of Agriculture and Animal Sciences,Huhhot 010030,China) |
| Abstract: | The BMPR-IB gene is the firstly discovered gene which the mechanism on ovulation rate of ovine has been clarified.BMPR-IB gene was mainly expressed in reproductive tissues and organs and played an important role in regulation of ovulation in sheep.The current study examined expressional patterns of BMPR-IB gene in the ovarian and uterus tissues at different stages in Mongolian sheep by using relative quantitative Real-time Quantitative PCR(RQ-PCR).The results showed that the regression equation of β-Actin gene and BMPR-IB gene amplification curve were y=-3.358x+35.708 and y=-2.119x+31.424,regression coefficient R2=0.990/0.992,respectively.The calculated result indicated that the BMPR-IB gene was highest expressed in the ovary and lowest in the uterus of Mongolian sheep at the estrus stage,the expression of BMPR-IB gene at estrus stage was 2.65 times high compared with other stages. |
| Keywords: | Mongolian sheep Bone Morphogenetic Protein Receptor-IB Gene Differential Expression Real-time Quantitative PCR |
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