贵州地方猪BF基因的群体遗传特性分析

以BF基因为候选基因,利用PCR和直接测序技术对贵州白香猪、可乐猪、大约克猪3个品种共95个个体BF基因外显子15、16、17的733 bp进行多态性分析,并对不同品种进行群体遗传学分析。结果显示:在第16外显子45 bp处存在A→C的碱基突变,命名为BF-exon16-A45C,导致编码的氨基酸由谷氨酸(Glu)突变为天冬氨酸(Asp);在贵州白香猪和可乐猪群体内均检测到AA、AC和CC三种基因型,在大约克猪群体内只检测到AC和CC两种基因型,贵州白香猪处于低度多态,可乐猪和大约克猪处于中度多态。经χ2适合性检验表明:贵州白香猪在该位点偏离了Hardy-Weinberg遗传平衡状态,可乐猪和大约克猪均处于Hardy-Weinberg遗传平衡状态。

Study on genetic characters of BF gene in the breeds of Guizhou local pigs

Taking BF gene as one of the candidate genes in present study,the polymorphism of exon 15~17 of BF gene about 733 bp in 95 individuals from Guizhou white pig,Kele pig and Large Yorkshire were analyzed with the method of PCR and direct sequencing,and analyzed the population genetic in different species.The results showed that there was a transversion at nucleotide position 45 of BF gene in the 16th exon named as BF-exon16-A45C,which led to a conservative Glutamic-to-Aspartic substitution at amino acid position 14;the three genotypes of AA,AC,CC were detected in Guizhou White pig and Kele pig,but in Yorkshire sows only two genotypes of AC and CC were detected,and Guizhou White pig in low polymorphism,which coke pig and Large Yorkshire at a moderate polymorphism.The frequencies of all pattern types got fitted Hardy-Weinberg equilibrium with χ2-fitness test in Kele pig and Large Yorkshire,while the same results did not appear in Guizhou White pig.