广西猪繁殖与呼吸综合征病毒感染状况调查

采用RT-PCR技术,对2004年1月至2005年4月期间,广西13个市104个疑似猪繁殖与呼吸综合征病毒(PRRSV)感染猪场,无菌采取231头病、死猪的组织病料(肺脏、淋巴结、脾脏)进行了病毒检测。同时,对鉴定为PRRSV阳性的组织病料和猪场进行了猪圆环病毒2型(PCV2)、猪瘟病毒(CSFV)和猪伪狂犬病毒(PRV)的检测,以确定猪群中PRRSV与PCV2、CSFV和PRV混合感染情况。结果从12个市的115份组织病料中检出PRRSV,病料的平均阳性率为49.78%(115/231),猪场的平均阳性率为61.54%(64/104),不同地市有一定的差异。PRRSV与PCV2、CSFV和/或PRV二重或多重混和感染的组织病料总数为53份,猪场总数为39个,混合感染的组织病料和猪场的总阳性率分别为22.94%(53/231)和37.50%(39/104)。混合感染的组织病料占PRRSV阳性组织病料的46.09%(53/115),混合感染的猪场占PRRSV阳性猪场的60.94%(39/64)。其中以PCV2和PRRSV混合感染的组织病料和猪场数最多。由此可见,PRRSV感染在广西猪场已普遍存在,与其他病毒混合感染现象逐渐趋向复杂化。

Epidemiological investigation on the infection of porcine reproductive and respiratory syndrome virus in Guangxi

Two hundred thiryty one tissue samples from 104 different pig farms of 13 regions,recorded from January 2004 to April 2005,were detected for porcine reproductive and respiratory syndrome virus(PRRSV) by RT-PCR.Clinical samples positive for PRRSV were also tested for the presence of porcine circovirus type 2(PCV-2),classical swine fever virus(CSFV) and pseudorabies virus(PRV).The results were as follows: 12 regions were positive for PRRSV.Average positive rates of PRRSV in samples and pig farms were 49.78%(115/231)and 61.54%(64/104),respectively.Samples and pig farms with dual and multiple infection of PRRSV and PCV2,CSFV and/or PRV accounted for 22.94%(53/231)and 37.50%(39/104),respectively.Especially,the rate of co-infection with PRRSV and PCV2 was very high.The results indicated that PRRSV widely existed in Guangxi and the co-infection enhanced the severity of PRRSV infection.